Increased gene expression of scavenger receptors and proinflammatory markers in peripheral blood mononuclear cells of hyperlipidemic males

被引:16
作者
Bonaterra, Gabriel A.
Hildebrandt, Wulf
Bodens, Anne
Sauer, Roland
Dugi, Klaus A.
Deigner, Hans-Peter
Turcanu, Dan
Heinle, Helmut
Droege, Wulf
Metz, Jurgen
Kinscherf, Ralf
机构
[1] Univ Heidelberg, Dept Anat & Cell Biol 3, D-69120 Heidelberg, Germany
[2] German Canc Res Ctr, Heidelberg, Germany
[3] Univ Heidelberg, Dept Internal Med 1, Heidelberg, Germany
[4] Univ E Anglia, Sch Chem Sci & Pharm, Norwich, Norfolk, England
[5] Univ Tubingen, Dept Physiol 1, Tubingen, Germany
来源
JOURNAL OF MOLECULAR MEDICINE-JMM | 2007年 / 85卷 / 02期
关键词
arteriosclerosis; hyperlipidemia; inflammatory genes; oxLDL; peripheral blood mononuclear cells;
D O I
10.1007/s00109-006-0117-6
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Interactions between peripheral blood mononuclear cells (PBMCs) and those within plaques are suggested to be pathophysiologically relevant to lipid-induced arteriosclerosis. In this study, gene expressions of scavenger receptors (CD36, CD68), LPS receptor (CD14), proinflammatory (tumor necrosis factor alpha [TNF alpha], CD40, interleukin-1 beta [IL-1 beta]) and oxidative stress-related (manganese superoxide dismutase [MnSOD]) markers were analyzed in PBMCs of clinically asymptomatic males with classical proatherogenic risk factors such as smoking and/or hyperlipidemia. PBMCs were isolated from venous blood of normolipidemic non-smokers (n=10) and smokers (n=8), and hyperlipidemic non-smokers (n=9) and smokers (n=8). RNA from PBMCs was used for PCR analyses. Plasma concentrations of oxidized low-density lipoproteins (oxLDL) were measured by ELISA. The gene expressions of CD36, CD68, CD40, TNF alpha, and MnSOD were significantly higher in PBMCs of hyperlipidemics than in normolipidemics, irrespective of whether they were smoking or not. The individual expression of these genes showed significant positive correlations with each other but also with serum cholesterol or plasma oxLDL concentrations. The higher expressions of scavenger receptors, proinflammatory and oxidative stress-related genes of PBMCs are suggested to result mainly from hyperlipidemia and the accompanied increase of oxLDL concentrations.
引用
收藏
页码:181 / 190
页数:10
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