Assessment of toxic potential of industrial chemicals using a cultured human bioartificial skin model:: Production of interleukin 1α and hydroxyeicosatetraenoic acids

被引:16
作者
Yang, EK [1 ]
Yoon, HH [1 ]
Lee, DH [1 ]
Park, JK [1 ]
机构
[1] Dongguk Univ, Dept Chem Engn, Lab Tissue Engn, Chung Gu, Seoul 100715, South Korea
来源
SKIN PHARMACOLOGY AND APPLIED SKIN PHYSIOLOGY | 2000年 / 13卷 / 05期
关键词
artificial skin; cutaneous toxicity; interleukin-1 alpha (lL-1 alpha); hydroxyeicosatetraenoic acid (HETE); proinflammatory mediator;
D O I
10.1159/000029930
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Cytotoxicity assays using artificial skin are proposed as alternative methods for in vitro tests to minimize animals used in ocular and dermal irritation testing. The responses of the artificial skins were studied to a well-characterized chemical irritant, such as toluene, glutaraldehyde and sodium lauryl sulfate (SLS), and a nonirritant, such as polyethylene glycol. The evaluation of irritating and nonirritating test chemicals was also compared with responses seen in human dermal fibroblasts and human epidermal keratinocytes grown in monolayer culture. The responses monitored included the MTT mitochondrial functionality assay. In order to bet-ter understand the local mechanisms involved in skin damage and repair, the productions of several mitogenic pro-inflammatory mediators such as interleukin-1 alpha (IL-1 alpha), 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE were investigated. Dose-dependent increases in the levels of IL-1 alpha and HETEs were observed in the underlying medium of the skin systems exposed to two skin irritants, glutaraldehyde and SLS. The results of the present study show that both human artificial skins can be used as efficient testing models for the evaluation of skin toxicity in vitro and for screening the contact skin irritancy in vitro, Copyright (C) 2000 S. Karger AG, Basel.
引用
收藏
页码:246 / 257
页数:12
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