Crystallization and preliminary crystallographic analysis of yeast cytosine deaminase

被引：2

作者：

Hsu, YH

Hu, CY

Lin, JJ

Liaw, SH ^{[1
]}

机构：

[1] Natl Yang Ming Univ, Inst Biochem, Taipei 112, Taiwan

[2] Natl Yang Ming Univ, Inst Biopharmaceut Sci, Taipei 112, Taiwan

[3] Natl Yang Ming Univ, Dept Life Sci, Taipei 112, Taiwan

[4] Taipei Vet Gen Hosp, Dept Med Res & Educ, Taipei, Taiwan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444903005936

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytosine deaminase is an attractive candidate for anticancer gene therapy through its catalysis of the deamination of the prodrug 5-fluorocytosine to 5-fluorouracil. Recombinant yeast cytosine deaminase has been crystallized with the inhibitor 2-hydroxypyrimidine in 10% 2-propanol, 20% polyethylene glycol 4000, 0.1 M HEPES pH 7.5. The crystals belong to space group P2(1), with unit-cell parameters a = 45.31, b = 53.33, c = 64.29 Angstrom, beta = 99.98degrees and one dimer per asymmetric unit. The crystals diffract X-rays beyond 1.5 Angstrom resolution and an initial atomic model has been built based on selenomethionyl multiwavelength anomalous data at 2 Angstrom resolution.

引用

页码：950 / 952

页数：3

共 16 条

[1] CYTIDINE DEAMINASE - THE 2-CENTER-DOT-3-ANGSTROM CRYSTAL-STRUCTURE OF AN ENZYME - TRANSITION-STATE ANALOG COMPLEX [J].