Biodiesel fuel production by the transesterification reaction of soybean oil using immobilized lipase

被引:59
作者
Bernardes, Otavio L.
Bevilaqua, Juliana V.
Leal, Marcia C. M. R.
Freire, Denise M. G.
Langone, Marta A. P.
机构
[1] Univ Estado Rio De Janeiro, Inst Quim, Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Inst Quim, BR-21949900 Rio De Janeiro, Brazil
关键词
enzyme; ethanol; immobilized lipase; methanol; solvent; soybean oil;
D O I
10.1007/s12010-007-9043-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyrne RM IM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best conditions were obtained in a solvent-free system with ethanol/oil molar ratio of 3.0, temperature of 50 degrees C, and enzyme concentration of 7.0% (w/w). Three-step batch ethanolysis was most effective for the production of biodiesel. Ethyl esters yield was about 60% after 4 h of reaction.
引用
收藏
页码:105 / 114
页数:10
相关论文
共 15 条
[1]   Prospects of biodiesel production from vegetables oils in India [J].
Barnwal, BK ;
Sharma, MP .
RENEWABLE & SUSTAINABLE ENERGY REVIEWS, 2005, 9 (04) :363-378
[2]   Efficient lipase catalysed production of a lubricant and surfactant formulation using a continuous solvent-free process [J].
Dossat, V ;
Combes, D ;
Marty, A .
JOURNAL OF BIOTECHNOLOGY, 2002, 97 (02) :117-124
[3]   Comparative study on lipase-catalyzed transformation of soybean oil for biodiesel production with different acyl acceptors [J].
Du, W ;
Xu, YY ;
Liu, DH ;
Zeng, J .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2004, 30 (3-4) :125-129
[4]  
ILLANES A, 1994, BIOTECNOLOGIA ENZIMA, P235
[5]   Production of biodiesel fuel from triglycerides and alcohol using immobilized lipase [J].
Iso, M ;
Chen, BX ;
Eguchi, M ;
Kudo, T ;
Shrestha, S .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2001, 16 (01) :53-58
[6]   Immobilized Candida antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium [J].
Köse, Ö ;
Tüter, M ;
Aksoy, HA .
BIORESOURCE TECHNOLOGY, 2002, 83 (02) :125-129
[7]  
KOSKINEN AMD, 1996, ENZYMATIC REACTIONS, P10
[8]   FACTORS AFFECTING THE ESTERIFICATION OF LAURIC ACID USING AN IMMOBILIZED BIOCATALYST - ENZYME CHARACTERIZATION AND STUDIES IN A WELL-MIXED REACTOR [J].
LIMA, FV ;
PYLE, DL ;
ASENJO, JA .
BIOTECHNOLOGY AND BIOENGINEERING, 1995, 46 (01) :69-79
[9]  
MEHER LC, 2004, RENEW SUST ENERG REV, V20, P1
[10]   Optimization of lipase-catalyzed biodiesel by response surface methodology [J].
Shieh, CJ ;
Liao, HF ;
Lee, CC .
BIORESOURCE TECHNOLOGY, 2003, 88 (02) :103-106