A green fluorescent chemosensor for amino acids provides a versatile high-throughput screening (HTS) assay for proteases

被引：83

作者：

Dean, KES ^{[1
]}

Klein, G ^{[1
]}

Renaudet, O ^{[1
]}

Reymond, JL ^{[1
]}

机构：

[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2003年 / 13卷 / 10期

关键词：

D O I：

10.1016/S0960-894X(03)00280-4

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The water soluble fluorescein-based ligand 1 forms a non-fluorescent complex with Cu2+. This complex serves as a fluorescent sensor for amino acids in the 10(-3) M Concentration range. Since the signal response is very fast, the sensor can be used to detect the hydrolytic activity of various proteases (trypsin, chymotrypsin, subtilisin) on bovine serum albumin as a whole protein substrate, and more generally to follow reactions releasing or removing free amino acids, in real time. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：1653 / 1656

页数：4

共 23 条

[1] Achieving large color changes in response to the presence of amino acids:: A molecular sensing ensemble with selectivity for aspartate
Aït-Haddou, H
Wiskur, SL
Lynch, VM
Anslyn, EV
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (45) : 11296 - 11297
[2] Highly photoresistant chemosensors using acridone as fluorescent label
Bahr, N
Tierney, E
Reymond, JL
[J]. TETRAHEDRON LETTERS, 1997, 38 (09) : 1489 - 1492
[3] Campbell A. M., 1991, MONCLONAL ANTIBODIES, P343
[4] Signaling recognition events with fluorescent sensors and switches
de Silva, AP
Gunaratne, HQN
Gunnlaugsson, T
Huxley, AJM
McCoy, CP
Rademacher, JT
Rice, TE
[J]. CHEMICAL REVIEWS, 1997, 97 (05) : 1515 - 1566
[5] Fabbrizzi L, 2002, ANGEW CHEM INT EDIT, V41, P3811, DOI 10.1002/1521-3773(20021018)41:20<3811::AID-ANIE3811>3.0.CO
[6] 2-W
[7] Fabbrizzi L, 2002, CHEM-EUR J, V8, P94, DOI 10.1002/1521-3765(20020104)8:1<94::AID-CHEM94>3.0.CO
[8] 2-L
[9] Geymayer P, 1999, CHEM-EUR J, V5, P1006, DOI 10.1002/(SICI)1521-3765(19990301)5:3<1006::AID-CHEM1006>3.0.CO
[10] 2-O

← 1 2 3 →