Induction of human osteoclast-like cells by treatment of blood monocytes with anti-fusion regulatory protein-1/CD98 monoclonal antibodies

被引：62

作者：

Higuchi, S ^{[1
]}

Tabata, N ^{[1
]}

Tajima, M ^{[1
]}

Ito, M ^{[1
]}

Tsurudome, M ^{[1
]}

Sudo, A ^{[1
]}

Uchida, A ^{[1
]}

Ito, Y ^{[1
]}

机构：

[1] Mie Univ, Sch Med, Dept Orthoped, Tsu, Mie, Japan

来源：

JOURNAL OF BONE AND MINERAL RESEARCH | 1998年 / 13卷 / 01期

关键词：

D O I：

10.1359/jbmr.1998.13.1.44

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have developed a nem and simple system of human osteoclast formation by fusing peripheral blood monocytes with anti-Fusion Regulatory Protein-1 (anti-FRP-1) monoclonal antibody (mAb). When human blood monocytes were cultured in the presence of anti-FRP-1/CD98 mAbs, polykaryocytes began to appear at approximately 15 h and increased in size with time until 3-4 days of incubation with anti-FRP-1 mAb. These fused cells showed positive staining in tartrate-resistant acid phosphatase, possessed numerous calcitonin receptors, and were capable of bone resorption, These results strongly suggest that anti-FRP-1 antibody-induced multinucleated cells are osteoclasts. Furthermore, FRP-1 antigens were detected in osteoclasts isolated from human bone and in the osteoclast-like cells obtained from human giant cell tumors of bone.

引用

页码：44 / 49

页数：6

共 21 条

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