Enforced detachment of red blood cells adhering to surfaces: Statics and dynamics

We investigated the mechanical strength of adhesion and the dynamics of unbinding of red blood cells to solid surfaces. Two different situations were tested: 1), native red blood cells nonspecifically adhered to glass surfaces coated with positively charged polymers and 2), biotinylated red blood cells specifically adhered to glass surfaces decorated with streptavidin, which has a high binding affinity for biotin. We used micropipette manipulation for forming and subsequently breaking the adhesive contact through a stepwise micromechanical procedure. Analysis of cell deformations provided the relation between force and contact radius, which was found to be in good agreement with theoretical predictions. We further demonstrated that the separation energy could be precisely derived from the measure of rupture forces and the cell shape. Finally, the dynamics of detachment was analyzed as a function of the applied force and the initial size of the adhesive patch. Our experiments were supported by original theoretical predictions, which allowed us to correlate the measured separation times with the molecular parameters (e.g., activation barrier, receptor-ligand characteristic length) derived from force measurements at the single bond level.