Quantitative proteomic assessment of very early cellular signaling events

被引：92

作者：

Dengjel, Joern

Akimov, Vyacheslav

Olsen, Jesper V.

Bunkenborg, Jakob

Mann, Matthias

Blagoev, Blagoy

Andersen, Jens S. ^{[1
]}

机构：

[1] Univ So Denmark, Ctr Expt Bioinformat, Dept Biochem & Mol Biol, DK-5230 Odense, Denmark

[2] Max Planck Inst Biochem, Dept Proteom & Signal Transduct, D-82152 Martinsried, Germany

来源：

NATURE BIOTECHNOLOGY | 2007年 / 25卷 / 05期

关键词：

D O I：

10.1038/nbt1301

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.

引用

页码：566 / 568

页数：3

共 16 条

[1] Mass spectrometry-based proteomics
Aebersold, R
Mann, M
[J]. NATURE, 2003, 422 (6928) : 198 - 207
[2] Nucleolar proteome dynamics
Andersen, JS
Lam, YW
Leung, AKL
Ong, SE
Lyon, CE
Lamond, AI
Mann, M
[J]. NATURE, 2005, 433 (7021) : 77 - 83
[3] Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics
Blagoev, B
Ong, SE
Kratchmarova, I
Mann, M
[J]. NATURE BIOTECHNOLOGY, 2004, 22 (09) : 1139 - 1145
[4] A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling
Blagoev, B
Kratchmarova, I
Ong, SE
Nielsen, M
Foster, LJ
Mann, M
[J]. NATURE BIOTECHNOLOGY, 2003, 21 (03) : 315 - 318
[5] COUNTAWAY JL, 1989, J BIOL CHEM, V264, P10828
[6] The Croonian Lecture 1997. The phosphorylation of proteins on tyrosine: Its role in cell growth and disease
Hunter, T
[J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1998, 353 (1368) : 583 - 605
[7] Signaling - 2000 and beyond
Hunter, T
[J]. CELL, 2000, 100 (01) : 113 - 127
[8] Quantification of short term signaling by the epidermal growth factor receptor
Kholodenko, BN
Demin, OV
Moehren, G
Hoek, JB
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (42) : 30169 - 30181
[9] Mechanism of divergent growth factor effects in mesenchymal stem cell differentiation
Kratchmarova, I
Blagoev, B
Haack-Sorensen, M
Kassem, M
Mann, M
[J]. SCIENCE, 2005, 308 (5727) : 1472 - 1477
[10] Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns
Larsen, MR
Thingholm, TE
Jensen, ON
Roepstorff, P
Jorgensen, TJD
[J]. MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (07) : 873 - 886

← 1 2 →