Glutamate receptor blockade attenuates glucose hypermetabolism in perihematomal brain after experimental intracerebral hemorrhage in rat

被引:54
作者
Ardizzone, TD
Lu, AG
Wagner, KR
Tang, Y
Ran, RQ
Sharp, FR
机构
[1] Univ Calif Davis, Med Ctr, MIND Inst, Sacramento, CA 95817 USA
[2] Univ Calif Davis, Dept Neurol, Sacramento, CA 95817 USA
[3] Univ Cincinnati, Coll Med, Dept Neurol, Cincinnati, OH 45221 USA
[4] Univ Cincinnati, Coll Med, Dept Pediat, Cincinnati, OH 45221 USA
[5] Univ Cincinnati, Coll Med, Grad Program Neurosci, Cincinnati, OH 45221 USA
[6] Dept Vet Affairs Med Ctr, Med Res Serv, Cincinnati, OH USA
关键词
intracerebral hemorrhage; MK-801; stroke; hemorrhagic;
D O I
10.1161/01.STR.0000143451.14228.ff
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Background and Purpose-Intracerebral hemorrhage has no effective treatment. The delayed appearance of edema, apoptosis, and inflammation in perihematomal brain suggests that these events may be targets for therapeutic intervention. To develop successful treatments, we must learn more about the effects of hemorrhage on brain tissue. In this study, we investigated the acute metabolic effects of intrastriatal hemorrhage in rat brain. Methods-Lysed blood or saline (50 muL each) was injected into the striatum of male Sprague-Dawley rats. The rats recovered for 1 to 72 hours before injection of [C-14]-2-deoxyglucose (intraperitoneally) 30 minutes before decapitation. Animals were pretreated with the N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) glutamate receptor antagonists dizolcilpine maleate (MK-801; 1 mg/kg) or 2,3-dihydroxy- 6-nitro-7-sulfamoylbenzo[f] quinoxaline (NBQX; 30 mg/kg), or saline vehicle. Additional animals received intrastriatal injections of glutamate (1.0 mmol/L), NMDA (1.0 mmol/L), or AMPA (0.1 mmol/L) in the place of blood. Semiquantitative autoradiographs from the brains were analyzed to determine the effects of hemorrhage on relative glucose metabolism. Results-We found an acute phase of increased [C-14]-2-deoxyglucose uptake in the perihematomal region that peaks 3 hours after lysed blood injection. Saline injections had no effect on striatal glucose utilization. The increased [C-14]-2-deoxyglucose uptake produced by the hemorrhages was blocked by pretreatment with MK-801 and NBQX. Glutamate injections alone had no effect on striatal metabolism, whereas NMDA and AMPA injections increased [C-14]-2-deoxyglucose uptake. Conclusions-The data imply that glutamate activation of NMDA or AMPA receptors increases glucose metabolism in perihematomal brain at early times after intracerebral hemorrhage. This may provide a possible target for the treatment of intracerebral hemorrhage.
引用
收藏
页码:2587 / 2591
页数:5
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