Atomic force microscopy examination of tobacco mosaic virus and virion RNA

被引：58

作者：

Drygin, YF ^{[1
]}

Bordunova, OA

Gallyamov, MO

Yaminsky, IV

机构：

[1] Moscow State Univ, AN Belozersky Inst Phys & Chem Biol, Moscow 119899, Russia

[2] Moscow State Univ, Fac Phys, Moscow 119899, Russia

[3] Moscow State Univ, Fac Chem, Moscow 119899, Russia

来源：

FEBS LETTERS | 1998年 / 425卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

atomic force microscopy; ribonucleoprotein; RNA; tobacco mosaic virus;

D O I：

10.1016/S0014-5793(98)00232-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Atomic force microscopy (AFM) was applied to study uncoated virus particles and RNA prepared by stripping of tobacco mosaic virions (TMV) with mild alkali or urea and dimethylsulfoxide. We found that AFM is an appropriate method to study ribonucleoprotein and free RNA structures. Images of entire tobacco mosaic virions, partially uncoated TMV particles with protruding RNA molecule from one or both ends and individual RNA molecules are presented. (C) 1998 Federation of European Biochemical Societies.

引用

页码：217 / 221

页数：5

共 21 条

[1] THE EFFECT OF UREA ON TOBACCO MOSAIC-VIRUS - POLARITY OF DISASSEMBLY
BLOWERS, LE
WILSON, TMA
[J]. JOURNAL OF GENERAL VIROLOGY, 1982, 61 (JUL) : 137 - 141
[2] BOEDTKER H, 1968, METHODS ENZYMOL B, V12, P429
[3] CIRCULAR DNA-MOLECULES IMAGED IN AIR BY SCANNING FORCE MICROSCOPY
BUSTAMANTE, C
VESENKA, J
TANG, CL
REES, W
GUTHOLD, M
KELLER, R
[J]. BIOCHEMISTRY, 1992, 31 (01) : 22 - 26
[4] DAVIS RW, 1971, METHOD ENZYMOL, V21, P413
[5] DRYGIN YF, 1997, INT NAN US C SANT BA, P39
[6] HIGH CONTRAST STAINING OF INDIVIDUAL NUCLEIC ACID MOLECULES
GORDON, CN
KLEINSCHMIDT, AK
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 155 (01) : 305 - +
[7] Atomic force microscopy of long and short double-stranded, single-stranded and triple-stranded nucleic acids
Hansma, HG
Revenko, I
Kim, K
Laney, DE
[J]. NUCLEIC ACIDS RESEARCH, 1996, 24 (04) : 713 - 720
[8] HOGUE R, 1984, CAN J BOT, V62, P2236
[9] STUDIES ON SECONDARY STRUCTURE OF SINGLE-STRANDED RNA FROM BACTERIOPHAGE-MS2 BY ELECTRON-MICROSCOPY
JACOBSON, AB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (02) : 307 - 311
[10] Escherichia coli RNA polymerase activity observed using atomic force microscopy
Kasas, S
Thomson, NH
Smith, BL
Hansma, HG
Zhu, XS
Guthold, M
Bustamante, C
Kool, ET
Kashlev, M
Hansma, PK
[J]. BIOCHEMISTRY, 1997, 36 (03) : 461 - 468

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