Contribution of IgH-PCR to the evaluation of B-cell lymphoma involvement in paraffin-embedded bone marrow biopsy specimens

被引：15

作者：

Braunschweig, R ^{[1
]}

Baur, AS ^{[1
]}

Delacrétaz, F ^{[1
]}

Bricod, C ^{[1
]}

Benhattar, J ^{[1
]}

机构：

[1] CHU Vaudois, Inst Pathol, CH-1011 Lausanne, Switzerland

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2003年 / 119卷 / 05期

关键词：

bone marrow biopsy; PCR; polymerase chain reaction; gene rearrangement; B cell; immunoglobulin heavy chain; single-strand conformation polymorphism;

D O I：

10.1309/NPQXQV82Q0R8VBKT

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

We investigated whether the determination of clonality by polymerase chain reaction (PCR) analysis of immunoglobulin heavy chain (IgH) gene rearrangements could be helpful in the evaluation of B-cell lymphoma (BCL) involvement of bone marrow (BM) biopsy specimens. We evaluated 83 paraffin-embedded BM biopsy specimens from 26 patients with BCL. When BM biopsy specimens considered positive, "suspicious," or negative by morphologic and immunohistochemical examination were evaluated by PCR, a monoclonal B-cell population was detected in 81% (39/48), 64% (9/14), and 11% (2/18), respectively. In most cases, a reproducible monoclonal IgH gene rearrangement was observed from BM and extramedullary sites. Nevertheless, in 4 cases, a different and independent monoclonal IgH rearrangement was observed during the disease course. PCR is efficient and complementary to morphologic and immunohistochemical examination for the evaluation of BCL involvement of BM biopsy specimens, especially when a reproducible rearrangement is found in 2 different samples.

引用

页码：634 / 642

页数：9

共 37 条

[11]

3.0.CO

[12]

2-Y

[13] Composite low grade B-cell lymphomas with two immunophenotypically distinct cell populations are true biclonal lymphomas -: A molecular analysis using laser capture microdissection [J].

Fend, F ;

Quintanilla-Martinez, L ;

Kumar, S ;

Beaty, MW ;

Blum, L ;

Sorbara, L ;

Jaffe, ES ;

Raffeld, M .

AMERICAN JOURNAL OF PATHOLOGY, 1999, 154 (06) :1857-1866

[14] DETECTION OF MONOCLONAL B-CELL POPULATIONS IN DECALCIFIED, PLASTIC-EMBEDDED BONE-MARROW BIOPSIES WITH THE POLYMERASE CHAIN-REACTION [J].

FEND, F ;

GSCHWENDTNER, A ;

GREDLER, E ;

THALER, J ;

DIETZE, O .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1994, 102 (06) :850-855

[15]

Finch CN, 2000, ARCH PATHOL LAB MED, V124, P1816

[16] MOLECULAR PATHOLOGY OF AIDS-RELATED LYMPHOMAS - BIOLOGIC ASPECTS AND CLINICOPATHOLOGICAL HETEROGENEITY [J].

GAIDANO, G ;

PASTORE, C ;

LANZA, C ;

MAZZA, U ;

SAGLIO, G .

ANNALS OF HEMATOLOGY, 1994, 69 (06) :281-290

[17] Polymerase chain reaction in the diagnosis of T-cell lymphoma in paraffin-embedded bone marrow biopsies:: a comparative study [J].

Gebhard, S ;

Benhattar, J ;

Bricod, C ;

Meugé-Moraw, C ;

Delacrétaz, F .

HISTOPATHOLOGY, 2001, 38 (01) :37-44

[18] Detection of immunoglobulin heavy chain gene rearrangement by polymerase chain reaction in chronic active gastritis associated with Helicobacter pylori [J].

Hsi, ED ;

Greenson, JK ;

Singleton, TP ;

Siddiqui, J ;

Schnitzer, B ;

Ross, CW .

HUMAN PATHOLOGY, 1996, 27 (03) :290-296

[19]

INGHIRAMI G, 1993, LAB INVEST, V68, P746

[20]

Jaffe ES., 2001, PATHOLOGY GENETICS T

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