Nonsense mutations in the lsa-like gene in Enterococcus faecalis isolates susceptible to lincosamides and streptogramins A

被引：38

作者：

Dina, J ^{[1
]}

Malbruny, B ^{[1
]}

Leclercq, R ^{[1
]}

机构：

[1] CHU Caen, Microbiol Serv, F-14033 Caen, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2003年 / 47卷 / 07期

关键词：

D O I：

10.1128/AAC.47.7.2307-2309.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The lsa gene confers intrinsic resistance to lincosamides and streptogramins A in Enterococcus faecalis, probably by active efflux. The lsa-like genes of two clinical isolates of E. faecalis susceptible to lincosamides and dalfopristin contained mutations that produced premature termination codons. Revertant mutants were obtained by selection on agar plates containing clindamycin.

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页码：2307 / 2309

页数：3

相关论文

共 11 条

[1] SEQUENCE OF A STAPHYLOCOCCAL PLASMID GENE, VGA, ENCODING A PUTATIVE ATP-BINDING PROTEIN INVOLVED IN RESISTANCE TO VIRGINIAMYCIN A-LIKE ANTIBIOTICS [J].

ALLIGNET, J ;

LONCLE, V ;

ELSOLH, N .

GENE, 1992, 117 (01) :45-51

[2] Effects of genes encoding resistance to streptogramins A and B on the activity of quinupristin-dalfopristin against Enterococcus faecium [J].

Bozdogan, B ;

Leclercq, R .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (11) :2720-2725

[3] In vitro activity of 19 antimicrobial agents against enterococci from healthy subjects and hospitalized patients and use of an ace gene probe from Enterococcus faecalis for species identification [J].

Duh, RW ;

Singh, KV ;

Malathum, K ;

Murray, BE .

MICROBIAL DRUG RESISTANCE, 2001, 7 (01) :39-46

[4] DETECTION OF GLYCOPEPTIDE RESISTANCE GENOTYPES AND IDENTIFICATION TO THE SPECIES LEVEL OF CLINICALLY RELEVANT ENTEROCOCCI BY PCR [J].

DUTKAMALEN, S ;

EVERS, S ;

COURVALIN, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (01) :24-27

[5] IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM HUMAN INFECTIONS BY A CONVENTIONAL TEST SCHEME [J].

FACKLAM, RR ;

COLLINS, MD .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (04) :731-734

[6] The Escherichia coli ATP-binding cassette (ABC) proteins [J].

Linton, KJ ;

Higgins, CF .

MOLECULAR MICROBIOLOGY, 1998, 28 (01) :5-13

[7] COMPARATIVE INVITRO ACTIVITY OF ERYTHROMYCIN, VANCOMYCIN AND PRISTINAMYCIN [J].

MASKELL, JP ;

SEFTON, AM ;

YONG, J ;

CHI, SJ ;

WILLIAMS, JD .

INFECTION, 1988, 16 (06) :365-370

[8] INDUCIBLE ERYTHROMYCIN RESISTANCE IN STAPHYLOCOCCI IS ENCODED BY A MEMBER OF THE ATP-BINDING TRANSPORT SUPERGENE FAMILY [J].

ROSS, JI ;

EADY, EA ;

COVE, JH ;

CUNLIFFE, WJ ;

BAUMBERG, S ;

WOOTTON, JC .

MOLECULAR MICROBIOLOGY, 1990, 4 (07) :1207-1214

[9] Minimal functional system required for expression of erythromycin resistance by msrA in Staphylococcus aureus RN4220 [J].

Ross, JI ;

Eady, EA ;

Cove, JH ;

Baumberg, S .

GENE, 1996, 183 (1-2) :143-148

[10] An Enterococcus faecalis ABC homologue (Lsa) is required for the resistance of this species to clindamycin and quinupristin-dalfopristin [J].

Singh, KV ;

Weinstock, GM ;

Murray, BE .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (06) :1845-1850