Is the nitric oxide system involved in genetic hypertension in Dahl rats?

被引:22
作者
Deng, AY [1 ]
机构
[1] Med Coll Ohio, Dept Physiol & Mol Med, Block Hlth Sci Bldg,3035 Arlington Ave, Toledo, OH 43699 USA
关键词
nitric oxide synthases; blood pressure; genetic hypertension; Dahl salt sensitive rat; quantitative trait locus; congenic strains;
D O I
10.1046/j.1523-1755.1998.00933.x
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
To address this issue, a series of genetic tests were carried out. Linkage studies showed that the inducible nitric oxide synthase (Nos2) locus cosegregated with blood pressure in three F-2 populations originated from crosses of Dahl salt-sensitive (S) rats with rats of various normotensive strains. However, the brain nitric oxidase synthase (Nos1) and endothelial nitric oxide synthase (Nos3) loci did not cosegregate with blood pressure in five F-2 populations. Thus, only Nos2, but not Nos1 and Nos3, was considered as a candidate gene for being a quantitative trait locus (QTL) for blood pressure in the S rat. To further test this hypothesis, congenic strains were constructed by substituting regions on Chromosome 10 of the S rat with the homologous regions of the Milan normotensive (MNS) rat. Results showed that the chromosome region including Nos2 did not contain a blood pressure QTL. In consequence, Nos2 per se is not supported as a candidate QTL capable of causing a blood pressure difference between the S and MNS rats. Nevertheless, the nitric oxide system appears to be involved secondarily in the regulation of blood pressure in the S rat, as evidenced by physiological data.
引用
收藏
页码:1501 / 1511
页数:11
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