Molecular methods for the detection and identification of Pseudomonas stutzeri in pure culture and environmental samples

被引:35
作者
Bennasar, A [1 ]
Guasp, C
Lalucat, J
机构
[1] Univ Illes Balears, Dept Biol Ambiental, Palma de Mallorca 07071, Spain
[2] UIB, CSIC, Inst Mediterrani Estudis Avancats, Palma de Mallorca 07071, Spain
关键词
D O I
10.1007/s002489900057
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
A polymerase chain reaction and an oligonucleotide probe method were developed for the specific detection and identification of the Gram-negative bacterium Pseudomonas stutzeri. This is an important species in environmental studies and can also be present in clinical samples. The primers for the amplification and the probe were designed from the analysis of 16S rRNA sequences. The PCR/oligonucleotide probe method, in combination with a direct lysis DNA extraction and an effective purification method, is an alternative to viable methods for the detection of P. stutzeri in environmental samples.
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收藏
页码:22 / 33
页数:12
相关论文
共 41 条
[1]   A METHOD FOR COUNTING DENITRIFIERS BY N2O DETECTION [J].
ALLIEVI, L ;
CATELANI, D ;
FERRARI, A ;
TRECCANI, V .
JOURNAL OF MICROBIOLOGICAL METHODS, 1987, 7 (2-3) :67-72
[2]   rRNA-targeted oligonucleotide probes for the identification of genuine and former pseudomonads [J].
Amann, R ;
Ludwig, W ;
Schulze, R ;
Spring, S ;
Moore, E ;
Schleifer, KH .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (04) :501-509
[3]  
[Anonymous], 1991, MANUAL CLIN MICROBIO
[4]  
[Anonymous], 1990, METHOD ENZYMOL
[5]  
BAWENS M, 1981, FLAVOBACTERIUM CYTOP, P27
[6]   16S rRNA gene sequence analysis relative to genomovars of Pseudomonas stutzeri and proposal of Pseudomonas balearica sp nov [J].
Bennasar, A ;
RosselloMora, R ;
Lalucat, J ;
Moore, ERB .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1996, 46 (01) :200-205
[7]  
BROSIUS J, 1978, P NATL ACAD SCI USA, V75, P4801, DOI 10.1073/pnas.75.10.4801
[8]  
CERNIGLIA E, 1984, PETROLEUM MICROBIOLO, P99
[9]  
CUYPERS H, 1992, PSEUDOMONAS : MOLECULAR BIOLOGY AND BIOTECHNOLOGY, P188
[10]  
Felsenstein J., 2005, PHYLIP PHYLOGENY INF, DOI DOI 10.1111/J.1096-0031.1989.TB00562.X