Quantification of endogenous steroids in human urine by gas chromatography mass spectrometry using a surrogate analyte approach

被引：30

作者：

Ahmadkhaniha, Reza ^{[4
,5
]}

Shafiee, Abbas ^{[4
,5
]}

Rastkari, Noushin ^{[3
]}

Khoshayand, Mohammad Reza ^{[2
]}

Kobarfard, Farzad ^{[1
]}

机构：

[1] Shahid Beheshti Univ, Sch Pharm, Dept Med Chem, Tehran, Iran

[2] Univ Tehran Med Sci, Fac Pharm, Dept Food & Drug Control, Tehran 14174, Iran

[3] Univ Tehran Med Sci, Environm Res Ctr, Tehran 14174, Iran

[4] Univ Tehran Med Sci, Fac Pharm, Dept Med Chem, Tehran 14174, Iran

[5] Univ Tehran Med Sci, Pharmaceut Sci Res Ctr, Tehran 14174, Iran

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2010年 / 878卷 / 11-12期

基金：

美国国家科学基金会;

关键词：

Endogenous steroid; Surrogate analyse; Calibration curve; Urine; Gas chromatography mass spectrometry; BIOLOGICAL SAMPLES; METHOD VALIDATION; DOPING CONTROL; HUMAN-SERUM; EXTRACTION; CORTISOL; HORMONES; PLASMA; MATRIX; MEAT;

D O I：

10.1016/j.jchromb.2010.01.040

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Providing "real blank sample" is a problem in determination of endogenous steroids in complex matrices. A new quantification strategy is proposed in the present study, which is based on using isotope-labeled steroids instead of natural steroids for constructing calibration line. This approach is called surrogate analyte and it is shown that its accuracy is better than some of the previously described methods at low concentrations and comparable to standard addition method at medium and high concentration levels. The method was fully validated to satisfy the ICH criteria and it was applied for determination of endogenous steroids in several urine samples. (c) 2010 Elsevier B.V. All rights reserved.

引用

页码：845 / 852

页数：8

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