Modeling spectral tuning in monomeric teal fluorescent protein mTFP1

被引：15

作者：

Topol, Igor ^{[1
]}

Collins, Jack ^{[1
]}

Nemukhin, Alexander ^{[2
,3
]}

机构：

[1] SAIC Frederick Inc, Adv Biomed Comp Ctr, Informat Syst Program, NCI Frederick, Ft Detrick, MD 21702 USA

[2] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia

[3] Russian Acad Sci, NM Emanuel Inst Biochem Phys, Moscow 119334, Russia

来源：

BIOPHYSICAL CHEMISTRY | 2010年 / 149卷 / 03期

基金：

俄罗斯基础研究基金会; 美国国家卫生研究院;

关键词：

Teal fluorescent protein mTFP1; Photoabsorption spectra; Quantum calculations; Mutation influence; GREEN; CHROMOPHORE; COLOR; GFP;

D O I：

10.1016/j.bpc.2010.04.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present results of theoretical studies of the variants of the monomeric teal fluorescent protein from Clavularia coral (mTFP1) which present promising members from the GFP family. Predictions of quantum chemical approaches including density functional theory and semiempirical approximations are presented for the model systems which mimic the chromophores in different environments We describe the excitation energy spectrum of the cyan mTFP1 fluorescent protein with the original chromophore and with chromophore mutants Tyr67His and Tyr67Trp (C) 2010 Elsevier B.V All rights reserved

引用

页码：78 / 82

页数：5

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