A group of expressed cDNA sequences from the wheat fungal leaf blotch pathogen, Mycosphaerella graminicola (Septoria tritici)

被引:45
作者
Keon, J [1 ]
Bailey, A [1 ]
Hargreaves, J [1 ]
机构
[1] Univ Bristol, Long Ashton Res Stn, IACR, Bristol BS41 9AF, Avon, England
关键词
cDNAs; expressed sequence tags; Mycosphaerella graminicola; plant pathogen; wheat;
D O I
10.1006/fgbi.2000.1186
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A group of expressed sequence tags (ESTs) from the wheat fungal pathogen Mycosphaerella graminicola utilizing ammonium as a nitrogen source has been analyzed, Single pass sequences of complementary DNAs from 986 clones were determined. Contig analysis and sequence comparisons allowed 704 unique ESTs (unigenes) to be identified, of which 148 appeared as multiple copies. Searches of the nrdb95 protein database at EMBL using the BLAST2x algorithm revealed 407 (57.8%) sequences that generated high to moderate high scoring pairs with proteins of known and unknown function. The rest of the sequences (297) showed either weak or no similarities to database entries. Among the unigenes with assigned function, 26.7% were involved in primary metabolism and 17.9% were associated with protein and RNA metabolism. Fewer clones were ascribed roles in signal transduction (4.9%), transport and secretion (6.1%), cell structure (3.1%), and cell division (3.6%). Approximately 18.1% of the identities found were to hypothetical or unknown proteins mainly from the yeasts Saccharomyces cerevisiae and Schizosaccaromyces pombe, Comparison of the 297 sequences with no clear function to other fungal ESTs in the public domain revealed 12 sequences that had high to moderate similarity to Neurospora crassa, Emericella (Aspergillus) nidulans, or Magnaporthe grisea sequences. (C) 2000 Academic Press.
引用
收藏
页码:118 / 133
页数:16
相关论文
共 20 条
  • [1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [2] ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
  • [3] Starvation-induced genes of the tomato pathogen Cladosporium fulvum are also induced during growth in planta
    Coleman, M
    Henricot, B
    Arnau, J
    Oliver, RP
    [J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 1997, 10 (09) : 1106 - 1109
  • [4] Cook RJ, 1999, SEPTORIA ON CEREALS: A STUDY OF PATHOSYSTEMS, P286
  • [5] Dancer J, 1999, SEPTORIA ON CEREALS: A STUDY OF PATHOSYSTEMS, P316
  • [6] Eyal Z, 1999, SEPTORIA ON CEREALS: A STUDY OF PATHOSYSTEMS, P1
  • [7] GURR SJ, 1992, MOL PLANT PATHOL, V1, P109
  • [8] A homologue of a gene implicated in the virulence of human fungal diseases is present in a plant fungal pathogen and is expressed during infection
    Hall, N
    Keon, JPR
    Hargreaves, JA
    [J]. PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1999, 55 (01) : 69 - 73
  • [9] Removing near-neighbour redundancy from large protein sequence collections
    Holm, L
    Sander, C
    [J]. BIOINFORMATICS, 1998, 14 (05) : 423 - 429
  • [10] Histology of the pathogenesis of Mycosphaerella graminicola in wheat
    Kema, GHJ
    Yu, DZ
    Rijkenberg, FHJ
    Shaw, MW
    Baayen, RP
    [J]. PHYTOPATHOLOGY, 1996, 86 (07) : 777 - 786