Sensitive analysis and pharmacokinetic study of the isomers paeoniflorin and albiflorin after oral administration of Total Glucosides Of White Paeony Capsule in rats

被引：43

作者：

Fei, Fei ^{[1
]}

Yang, Hailing ^{[2
]}

Peng, Ying ^{[1
]}

Wang, Pei ^{[1
]}

Wang, Shuyao ^{[3
]}

Zhao, Yuqing ^{[1
]}

Huang, Jingqiu ^{[1
]}

Yu, Xiaoyi ^{[1
]}

Feng, Siqi ^{[1
]}

Sun, Runbin ^{[1
]}

Yang, Na ^{[1
]}

Wang, Hongbo ^{[4
]}

Aa, Jiye ^{[1
]}

Wang, Guangji ^{[1
]}

机构：

[1] China Pharmaceut Univ, State Key Lab Nat Med, Jiangsu Key Lab Drug Design & Optimizat, Jiangsu Prov Key Lab Drug Metab & Pharmacokinet, Tongjiaxiang 24, Nanjing 210009, Jiangsu, Peoples R China

[2] Lansen Pharmaceut Holdings Co Ltd, Ningbo 315174, Zhejiang, Peoples R China

[3] Beijing Univ Chinese Med, Sch Chinese Pharm, Beijing 100102, Peoples R China

[4] Yantai Univ, Sch Pharm, Collaborat Innovat Ctr Adv Drug Delivery Syst & B, Yantai 264005, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2016年 / 1022卷

关键词：

LC-MS/MS; Albiflorin; Paeoniflorin; Pharmacokinetics; Tissue distribution; RADIX PAEONIAE ALBA; UPLC-MS/MS; RHEUMATOID-ARTHRITIS; DECOCTION; PLASMA; LACTIFLORA; CONSTITUENTS; EXTRACT; SERUM; RUBRA;

D O I：

10.1016/j.jchromb.2016.04.005

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

A sensitive and reliable method using liquid chromatography tandem mass spectrometry (LC-MS/MS) was established for the simultaneous assay of paeoniflorin and albiflorin in bio-samples of rats after liquid-liquid extraction with ethylacetate. For the first time, the developed method was validated and successfully applied to the pharmacokinetics study of paeoniflorin and albiflorin after oral administration of Total Glucosides Of White Paeony Capsule (TGP). Relative to the intravenous injection, the absolute bio-availabilities of paeoniflorin and albiflorin were 2.8 and 1.7%, while their excretion in feces was 43.06 and 40.87%, respectively. Both paeoniflorin and albiflorin showed dose-dependent exposure in plasma, with a half-life of approximately 1.8 h. No significant differences were observed between a single equal dose of paeoniflorin or albiflorin and that of TGP for the pharmacokinetic parameters, including AUC, T1/2 and Cmax. Paeoniflorin and albiflorin were exposed at high levels in immune relevant organ/tissues, such as the spleen, thymus and bone, which could facilitate immuno-regulatory activities. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：30 / 37

页数：8

共 18 条

[1]

Pharmacokinetic study of paeoniflorin in mice after oral administration of Paeoniae radix extract [J].