Xanthine oxidoreductase is asymmetrically localised on the outer surface of human endothelial and endothelial cells in culture

被引:57
作者
Rouquette, M
Page, S
Bryant, R
Benboubetra, M
Stevens, CR
Blake, DR
Whish, WD
Harrison, R [1 ]
Tosh, D
机构
[1] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England
[2] Univ Bath, Dept Postgrad Med, Bath BA2 7AY, Avon, England
基金
英国生物技术与生命科学研究理事会;
关键词
xanthine oxidoreductase; immunolocalization; endothelial; epithelial; human;
D O I
10.1016/S0014-5793(98)00385-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Subcellular localisation of xanthine oxidoreductase (XOR) was determined by indirect immunofluorescence using confocal microscopy in human endothelial and epithelial cell lines and in primary cultures of human umbilical vein endothelial cells. XOR was diffusely distributed throughout the cytoplasm but with higher intensity in the perinuclear region. In non-permeabilised cells, XOR was clearly seen to be asymmetrically located on the outer surfaces, showing, in many cases, a higher intensity on those faces apposed by closely neighbouring cells. Such specific distribution suggests a functional role for the enzyme in cell-cell interactions, possibly involving signalling via reactive oxygen species. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:397 / 401
页数:5
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