Occurrence of a cytosolic neutral chitobiase activity involved in oligomannoside degradation: A study with Madin-Darby bovine kidney (MDBK) cells

被引：45

作者：

Cacan, R ^{[1
]}

Dengremont, C ^{[1
]}

Labiau, O ^{[1
]}

Kmiecik, D ^{[1
]}

Mir, AM ^{[1
]}

Verbert, A ^{[1
]}

机构：

[1] UNIV SCI & TECH LILLE FLANDRES ARTOIS,CHIM BIOL LAB,CNRS,UMR 111,F-59655 VILLENEUVE DASCQ,FRANCE

来源：

BIOCHEMICAL JOURNAL | 1996年 / 313卷

关键词：

D O I：

10.1042/bj3130597

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neutral oligomannosides possessing one GlcNAc (OS-Gn1) and two GlcNAc (Os-Gn2) at the reducing end have been reported to be released during the N-glycosylation process in various biological models. To investigate which enzyme is responsible for OS-Gn1 formation, we used the Madin-Darby bovine kidney (MDBK) cell line which exhibits neither lysosomal chitobiase nor endoglucosaminidase activities. However, these cells produced OS-Gn1 and we showed that a neutral chitobiase is responsible for the transformation of OS-Gn2 into OS-Gn1. Using streptolysin O-permeabilized MDBK cells, we demonstrated that this neutral chitobiase activity is located in the cytosolic compartment and is active on oligomannoside species released during the N-glycosylation process.

引用

页码：597 / 602

页数：6

共 23 条

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