Platelet, Not Endothelial, P-Selectin Expression Contributes to Generation of Immunity in Cutaneous Contact Hypersensitivity

被引:10
作者
Ludwig, Ralf J. [1 ,2 ]
Bergmann, Peri [5 ]
Garbaraviciene, Jurate [2 ,6 ]
von Stebut, Esther [5 ]
Radeke, Heinfried H. [3 ]
Gille, Jens [2 ]
Diehl, Sandra [2 ]
Hardt, Katja [2 ]
Henschler, Reinhard [4 ]
Kaufmann, Roland [2 ]
Pfeilschifter, Josef M. [3 ]
Boehncke, Wolf-Henning [2 ]
机构
[1] Med Univ Lubeck, Dept Dermatol, D-23538 Lubeck, Germany
[2] Clin Johann Wolfgang Goethe Univ, Dept Dermatol, German Red Cross Blood Donor Serv, Frankfurt, Germany
[3] Clin Johann Wolfgang Goethe Univ, Pharmazentrum Frankfurt ZAFES, German Red Cross Blood Donor Serv, Frankfurt, Germany
[4] Clin Johann Wolfgang Goethe Univ, Inst Transfus Med & Immune Haematol, German Red Cross Blood Donor Serv, Frankfurt, Germany
[5] Johannes Gutenberg Univ Mainz, Dept Dermatol, Mainz, Germany
[6] Vilnius State Univ, Clin Infect Dis, Fac Med, Vilnius, Lithuania
关键词
DEFICIENT; LIGAND; SKIN; INFLAMMATION; RECRUITMENT; PROTEIN-1; RECEPTORS; SECRETION; TRIGGERS; DISTINCT;
D O I
10.2353/ajpath.2010.081100
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Leukocyte extravasation is a prerequisite for host defense and autoimmunity alike. Detailed understanding of the tightly controlled and overlapping sequences of leukocyte extravasation might aid development of novel therapeutic strategies. Leukocyte extravasation is initiated by interaction of selectins with appropriate carbohydrate ligands. Lack of P-selectin expression leads to decreased contact hypersensitivity responses. Yet, it remains unclear if this is due to inhibition of leukocyte extravasation to the skin or due to interference with initial immune activation in lymph nodes. in line with previous data, we here report a decreased contact hypersensitivity response, induced by 2,4,-dinitrofluoro-benzene (DNFB), in P-selectin-deficient mice. Eliciting an immune reaction towards DNFB in wild-type mice, followed by adoptive transfer to P-selectin-deficient mice, had no impact on inflammatory response in recipients. This was significantly reduced in wild-type recipient mice adoptively transferred with DNFB immunity generated in P-selectin-deficient mice. To investigate if platelet or endothelial P-selectin was involved, mice solely lacking platelet P-selectin expression generated by bone marrow transplantation were used. Adoptive transfer of immunity from wild-type mice reconstituted with P-selectin-deficient bone marrow led to a decrease of inflammatory response. Comparing this decrease to the one observed using P-selectin-deficient mice, no differences were observed. Our observations indicate that platelet, not endothelial, P-selectin contributes to generation of immunity in DNFB-induced contact hypersensitivity. (Am J Pathol 2010, 176:1339-1345; DOI: 10.2353/ajpath.2010.081100)
引用
收藏
页码:1339 / 1345
页数:7
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