Necessity of a functional octadecanoic pathway for indole alkaloid synthesis by Catharanthus roseus cell suspensions cultured in an auxin-starved medium

被引:78
作者
Gantet, P [1 ]
Imbault, N [1 ]
Thiersault, M [1 ]
Doireau, P [1 ]
机构
[1] Univ Tours, Fac Sci & Tech, Lab Physiol Vegetale, F-37200 Tours, France
关键词
Catharanthus roseus; methyl jasmonate; octadecanoic pathway; periwinkle;
D O I
10.1093/oxfordjournals.pcp.a029360
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effect of methyl jasmonate (mJA), jasmonic acid and traumatic acid, derivatives of the octadecanoic pathway, on the production of alkaloids by cell suspension cultures of Catharanthus roseus L. (G) Don was investigated, Cells cultured in the presence of auxin (m-cells) did not accumulate alkaloids, The addition of exogenous mJA to m-cells restored the ability to produce alkaloids, In cells cultured in a 2,4-D-starved medium (p-cells), exogenous mJA greatly increased alkaloid production, Similar data were obtained for jasmonic acid, In contrast, traumatic acid had no effect on alkaloid production, The sensitivity of cell suspension cultures to exogenous mJA was restricted to the first four days of subculture corresponding to the active growth phase, whereas the alkaloid accumulation occurred only during the stationary phase of the subculture (days 6 to 10), When p-cells were treated with octadecanoic pathway inhibitors, the ability to produce alkaloids was strongly reduced, The addition of exogenous mJA always restored the ability to produce alkaloids, These data suggest that in response to auxin depletion, endogenous mJA could be produced and act by linking physiological events thus leading to alkaloid biosynthesis activation.
引用
收藏
页码:220 / 225
页数:6
相关论文
共 23 条
  • [1] METHYL JASMONATE VAPOR INCREASES THE DEVELOPMENTALLY CONTROLLED SYNTHESIS OF ALKALOIDS IN CATHARANTHUS AND CINCHONA SEEDLINGS
    AERTS, RJ
    GISI, D
    DECAROLIS, E
    DELUCA, V
    BAUMANN, TW
    [J]. PLANT JOURNAL, 1994, 5 (05) : 635 - 643
  • [2] 2,4-D AND ALKALOID ACCUMULATION IN PERIWINKLE CELL-SUSPENSIONS
    ARVY, MP
    IMBAULT, N
    NAUDASCHER, F
    THIERSAULT, M
    DOIREAU, P
    [J]. BIOCHIMIE, 1994, 76 (05) : 410 - 416
  • [3] Activation of phospholipase a by plant defense elicitors
    Chandra, S
    Heinstein, PF
    Low, PS
    [J]. PLANT PHYSIOLOGY, 1996, 110 (03) : 979 - 986
  • [4] LIPID-DERIVED SIGNALS THAT DISCRIMINATE WOUND-RESPONSIVE AND PATHOGEN-RESPONSIVE ISOPRENOID PATHWAYS IN PLANTS - METHYL JASMONATE AND THE FUNGAL ELICITOR ARACHIDONIC-ACID INDUCE DIFFERENT 3-HYDROXY-3-METHYLGLUTARYL-COENZYME-A REDUCTASE GENES AND ANTIMICROBIAL ISOPRENOIDS IN SOLANUM-TUBEROSUM L
    CHOI, D
    BOSTOCK, RM
    AVDIUSHKO, S
    HILDEBRAND, DF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) : 2329 - 2333
  • [5] The octadecanoid signalling pathway in plants mediates a response to ultraviolet radiation
    Conconi, A
    Smerdon, MJ
    Howe, GA
    Ryan, CA
    [J]. NATURE, 1996, 383 (6603) : 826 - 829
  • [6] PUTATIVE SITES OF CYTOKININ ACTION DURING THEIR ENHANCING EFFECT ON INDOLE ALKALOID ACCUMULATION IN PERIWINKLE CELL-SUSPENSIONS
    DECENDIT, A
    PETIT, G
    ANDREU, F
    DOIREAU, P
    MERILLON, JM
    RIDEAU, M
    [J]. PLANT CELL REPORTS, 1993, 12 (12) : 710 - 712
  • [7] SALICYLIC-ACID INHIBITS SYNTHESIS OF PROTEINASE-INHIBITORS IN TOMATO LEAVES INDUCED BY SYSTEMIN AND JASMONIC ACID
    DOARES, SH
    NARVAEZVASQUEZ, J
    CONCONI, A
    RYAN, CA
    [J]. PLANT PHYSIOLOGY, 1995, 108 (04) : 1741 - 1746
  • [8] DIETHYLDITHIOCARBAMIC ACID INHIBITS THE OCTADECANOID SIGNALING PATHWAY FOR THE WOUND INDUCTION OF PROTEINASE-INHIBITORS IN TOMATO LEAVES
    FARMER, EE
    CALDELARI, D
    PEARCE, G
    WALKERSIMMONS, K
    RYAN, CA
    [J]. PLANT PHYSIOLOGY, 1994, 106 (01) : 337 - 342
  • [9] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS
    GAMBORG, OL
    MILLER, RA
    OJIMA, K
    [J]. EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) : 151 - +
  • [10] Signaling in soybean phenylpropanoid responses - Dissection of primary, secondary, and conditioning effects of light, wounding, and elicitor treatments
    Graham, TL
    Graham, MY
    [J]. PLANT PHYSIOLOGY, 1996, 110 (04) : 1123 - 1133