Doxorubicin uptake and release from microgel thin films

被引：193

作者：

Serpe, MJ ^{[1
]}

Yarmey, KA ^{[1
]}

Nolan, CM ^{[1
]}

Lyon, LA ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

来源：

BIOMACROMOLECULES | 2005年 / 6卷 / 01期

关键词：

D O I：

10.1021/bm049455x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report investigations on the thermally regulated uptake and release of the chemotherapeutic drug doxorubicin from microgel thin films. A spin coating, layer-by-layer (scLbL) assembly approach was used to prepare thin films composed of thermoresponsive poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAmAAc) microgels by alternatively exposing a 3-aminopropyltrimethoxysilane (APTMS) functionalized glass substrate to polyanionic pNIPAm-AAc microgels and polycationic poly(allylamine hydrochloride) (PAH). Using this method, 10, 20, and 30 microgel layer films were constructed with uniform layer buildup, as confirmed by quartz crystal microgravimetry (QCM). The films were subsequently loaded with doxorubicin by cycling the temperature of the film in an aqueous doxorubicin solution between 25 and 50 degreesC. Release characteristics were then examined using UV-vis spectroscopy, which revealed temperature-dependent release properties.

引用

页码：408 / 413

页数：6

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