Nitric oxide inhibits complex I following AMPA receptor activation via peroxynitrite

被引：6

作者：

Araújo, IM

Verdasca, MJ

Ambrósio, AF

Carvalho, CM ^{[1
]}

机构：

[1] Univ Coimbra, Dept Zool, Ctr Neurosci & Cell Biol, P-3004517 Coimbra, Portugal

[2] Univ Coimbra, Fac Med, Ctr Ophthalmol, IBILI, P-3000354 Coimbra, Portugal

来源：

NEUROREPORT | 2004年 / 15卷 / 13期

关键词：

AMPA receptors; hippocampal neurons; mitochondrial activity; nitric oxide; peroxynitrite;

D O I：

10.1097/00001756-200409150-00003

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We investigated the role of nitric oxide (NO) on mitochondrial complexes activity, following short-term non-desensitizing activation of AMPA receptors with kainate (KA) plus cyclothiazide (CTZ), in cultured rat hippocampal neurons. In these conditions, we observed a decrease in the activity of mitochondrial complexes I, II/III, and IV. A selective neuronal nitric oxide synthase inhibitor, 7-Nitroindazole, prevented the decrease in the activity of mitochondrial complex 1, but not for the other complexes. Exposure to KA plus CTZ also increased cyclic GMP levels significantly, and led to increased levels of 3-nitrotyrosine, a biomarker for peroxynitrite production. Taken together, our results suggest that non-desensitizing activation of AMPA receptors causes inhibition of mitochondrial complex 1 via peroxynitrite.

引用

页码：2007 / 2011

页数：5

共 22 条

[1] Glutamate neurotoxicity is associated with nitric oxide-mediated mitochondrial dysfunction and glutathione depletion [J].