Prevention of intracellular oxidative stress to lens by pyruvate and its ester

被引:77
作者
Varma, SD [1 ]
Devamanoharan, PS [1 ]
Ali, AH [1 ]
机构
[1] Univ Maryland, Sch Med, Dept Ophthalmol, Baltimore, MD 21201 USA
关键词
oxygen radicals; cataract; eye lens; menadione; pyruvate; pyruvate-ester;
D O I
10.3109/10715769809065799
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pyruvate is a well-known scavenger of hydrogen peroxide (H2O2). Ln addition, it scavenges superoxide radical (O-2(.-)). However, evidence on its intracellular antioxidant function is meager at present Hence, we have examined the effectiveness of this metabolite and its ethyl ester against intracellular oxidative damage to the lens under organ culture. Menadione, a redoxcycling quinone, was used to generate the reactive oxygen species (ROS). It was found to inhibit lens metabolism as evidenced by a decrease of Am. Additionally, tissue oxidation was apparent by loss of glutathione (GSH), and increase in the level of oxidized glutathione (GSSG), coupled with increase of the urea soluble proteins (water insoluble). The overall physiological damage was apparent by the inhibition of the Na+-K+-ATPase dependent cation pump, as evidenced by a decreased rubidium transport. These deleterious effects were attenuated by pyruvate and ethyl-pyruvate. The later was found to be more effective.
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页码:131 / 135
页数:5
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