Influence of extracellular bactericidal agents on bacteria within macrophages

被引：26

作者：

Hamrick, TS ^{[1
]}

Diaz, AH ^{[1
]}

Havell, EA ^{[1
]}

Horton, JR ^{[1
]}

Orndorff, PE ^{[1
]}

机构：

[1] N Carolina State Univ, Coll Vet Med, Dept Microbiol Pathol & Parasitol, Raleigh, NC 27606 USA

来源：

INFECTION AND IMMUNITY | 2003年 / 71卷 / 02期

关键词：

D O I：

10.1128/IAI.71.2.1016-1019.2003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We employed gentamicin-sensitive and -resistant derivatives of Escherichia coli in a macrophage phagocytosis assay that compared lambda bacteriophage and gentamicin as extracellullar bactericidal agents. Colony counts and direct microscopic examination of phagocytized E. coli supported the conclusion that gentamicin entered macrophages, even at low concentrations, and contributed to their bactericidal activity. Also, two E. coli strains differing in the ability to express the adhesin of type 1 pili (FimH) were distinguishably different in intracellular survival when lambda was used as the extracellular killing agent but were indistinguishable when gentamicin was employed.

引用

页码：1016 / 1019

页数：4

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