CD8+ T cells responding to influenza infection reach and persist at higher numbers than CD4+ T cells independently of precursor frequency

被引:34
作者
Powell, TJ [1 ]
Brown, DM [1 ]
Hollenbaugh, JA [1 ]
Charbonneau, T [1 ]
Kemp, RA [1 ]
Swain, SL [1 ]
Dutton, RW [1 ]
机构
[1] Trudeau Inst, Saranac Lake, NY 12983 USA
关键词
virus infection; T lymphocytes; cellular activation; cellular proliferation; cell surface molecules;
D O I
10.1016/j.clim.2004.05.006
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The activation, localization, phenotypic changes, and function of CFSE-labeled naive influenza-specific CD8(+) and CD4(+) T cells following influenza infection were examined. Response of adoptively transferred CD8(+) T cells was seen earliest in draining lymph node. Highly activated cells were found later in the lung, airways, and spleen, were cytolytic, and expressed IFN-gamma upon restimulation. Similar amounts of division at early time points, but higher numbers of CD8(+) T cells, were detected at 9 and 30 days postinfection after cotransfer of CD4(+) and CD8(+) T cells followed by infection. Transfer of much smaller numbers of CD4(+) and CD8(+) T cells led to more extensive expansion but the same difference in final number between the two cell types. These studies demonstrate how CD8(+) and CD4(+) T cells respond to influenza at early time points postinfection and the differential kinetics of antigen-specific CD4(+) and CD8(+) T cells. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:89 / 100
页数:12
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