Methods for introducing morpholinos into the chicken embryo

被引:57
作者
Kos, R
Tucker, RP
Hall, R
Duong, TD
Erickson, CA
机构
[1] Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 95616 USA
[2] Univ Calif Davis, Dept Cell Biol & Human Anat, Davis, CA USA
关键词
morpholinos; chick embryos; delivery methods;
D O I
10.1002/dvdy.10254
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The use of antisense morpholino oligos to inhibit the translation of a target transcript has been applied recently to studies of the chicken embryo. In contrast to other developmental systems such as in frog, sea urchin, and zebrafish that permit the direct microinjection of morpholinos into a blastomere, square pulse electroporation is used to introduce fluorescently tagged morpholinos into specific populations of chick embryo cells in ovo. This article reviews the methods that have proven successful, the types of controls that are necessary when performing knockdowns of gene expression in the chick embryo, and discusses the limitations of the current technique, as well as directions for further research. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:470 / 477
页数:8
相关论文
共 25 条
[1]  
BronnerFraser M, 1996, METHOD CELL BIOL, V51, P61, DOI 10.1016/S0091-679X(08)60622-6
[2]  
Chapman SC, 2001, DEV DYNAM, V220, P284, DOI 10.1002/1097-0177(20010301)220:3<284::AID-DVDY1102>3.3.CO
[3]  
2-X
[4]   Morphant technology in model developmental systems [J].
Ekker, SC ;
Larson, JD .
GENESIS, 2001, 30 (03) :89-93
[5]  
Hagman U, 1999, VAR FODA, V51, P24
[6]  
HALL RJ, 2003, IN PRESS DEV BIOL
[7]   β-catenin signaling activity dissected in the early Xenopus embryo:: A novel antisense approach [J].
Heasman, J ;
Kofron, M ;
Wylie, C .
DEVELOPMENTAL BIOLOGY, 2000, 222 (01) :124-134
[8]   Morpholino oligos: Making sense of antisense? [J].
Heasman, J .
DEVELOPMENTAL BIOLOGY, 2002, 243 (02) :209-214
[9]  
Kos R, 2001, DEVELOPMENT, V128, P1467
[10]  
Le Douarin N, 1999, NEURAL CREST, P1