Identification of acrolein-conjugated protein in plasma of patients with brain infarction

被引：33

作者：

Yoshida, Madoka ^{[1
,2
]}

Higashi, Kyohei ^{[1
,2
]}

Jin, Lihua ^{[1
]}

Machi, Yoshiki ^{[1
]}

Suzuki, Takehiro ^{[3
,4
]}

Masuda, Akiko ^{[3
,4
]}

Dohmae, Naoshi ^{[3
,4
]}

Suganami, Akiko ^{[5
]}

Tamura, Yutaka ^{[5
]}

Nishimura, Kazuhiro ^{[1
]}

Toida, Toshihiko ^{[1
]}

Tomitori, Hideyuki ^{[6
]}

Kashiwagi, Keiko ^{[6
]}

Igarashi, Kazuei ^{[1
,2
]}

机构：

[1] Chiba Univ, Grad Sch Pharmaceut Sci, Chuo Ku, Chiba 2608675, Japan

[2] Chiba Univ, Amine Pharma Res Inst, Chuo Ku, Chiba 2600856, Japan

[3] RIKEN Adv Sci Inst, Biomol Characterizat Team, Wako, Saitama 3510198, Japan

[4] JST, CREST, Wako, Saitama 3510198, Japan

[5] Chiba Univ, Grad Sch Med, Chuo Ku, Chiba 2608670, Japan

[6] Chiba Inst Sci, Fac Pharm, Choshi, Chiba 2880025, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 391卷 / 02期

关键词：

Acrolein; Albumin; MP-lysine; LC-MS/MS; Brain infarction; Renal failure; HUMAN-SERUM-ALBUMIN; AMINE OXIDASE; RENAL-FAILURE; MARKERS; CELLS; CHROMATOGRAPHY; EXPRESSION; POLYAMINES; ACIDS;

D O I：

10.1016/j.bbrc.2009.12.049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is known that the level of protein-conjugated acrolein in plasma is a good marker of chronic renal failure and brain infarction. Thus, studies were carried out to determine which kinds of plasma proteins are conjugated with acrolein. It was found that acrolein was mainly conjugated with albumin. Tandem mass spectrometry analysis demonstrated that Lys-557 and Lys-560, located at the surface of domain III of albumin, were the major sites conjugated with acrolein. This is the first report to identify the amino acid residues in a protein modified by acrolein in vivo. It was found that conjugation of acrolein with albumin contributed to a decrease in the toxicity of acrolein. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1234 / 1239

页数：6

共 25 条

[1] ALTERATION OF RIBONUCLEOTIDE REDUCTASE IN APHIDICOLIN-RESISTANT MUTANTS OF MOUSE FM3A CELLS WITH ASSOCIATED RESISTANCE TO ARABINOSYLADENINE AND ARABINOSYLCYTOSINE [J].

AYUSAWA, D ;

IWATA, K ;

SENO, T .

SOMATIC CELL GENETICS, 1981, 7 (01) :27-42

[2]

Cohen S.S., 1998, GUIDE POLYAMINES

[3] SYNTHESIS OF A FLUORESCENT DERIVATIZING REAGENT, 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE, AND ITS APPLICATION FOR THE ANALYSIS OF HYDROLYSATE AMINO-ACIDS VIA HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

COHEN, SA ;

MICHAUD, DP .

ANALYTICAL BIOCHEMISTRY, 1993, 211 (02) :279-287

[4] Nε-(3-methylpyridinium) lysine, a major antigenic adduct generated in acrolein-modified protein [J].

Furuhata, A ;

Ishii, T ;

Kumazawa, S ;

Yamada, T ;

Nakayama, T ;

Uchida, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (49) :48658-48665

[5] Reactive oxygen species, cell signaling, and cell injury [J].

Hensley, K ;

Robinson, KA ;

Gabbita, SP ;

Salsman, S ;

Floyd, RA .

FREE RADICAL BIOLOGY AND MEDICINE, 2000, 28 (10) :1456-1462

[6] Relations of serum high-sensitivity C-reactive protein and interleukin-6 levels with silent brain infarction [J].

Hoshi, T ;

Kitagawa, K ;

Yamagami, H ;

Furukado, S ;

Hougaku, H ;

Hori, M .

STROKE, 2005, 36 (04) :768-772

[7]

IBERG N, 1986, J BIOL CHEM, V261, P3542

[8] Polyamines in renal failure [J].

Igarashi, K. ;

Ueda, S. ;

Yoshida, K. ;

Kashiwagi, K. .

AMINO ACIDS, 2006, 31 (04) :477-483

[9] Polyamines: Mysterious modulators of cellular functions [J].

Igarashi, K ;

Kashiwagi, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 271 (03) :559-564

[10] The molecular effects of acrolein [J].

Kehrer, JP ;

Biswal, SS .

TOXICOLOGICAL SCIENCES, 2000, 57 (01) :6-15

← 1 2 3 →