Packaging of small molecules into VP1-virus-like particles of the human polyomavirus JC virus

被引：44

作者：

Goldmann, C ^{[1
]}

Stolte, N ^{[1
]}

Nisslein, T ^{[1
]}

Hunsmann, G ^{[1
]}

Lüke, W ^{[1
]}

Petry, H ^{[1
]}

机构：

[1] German Primate Ctr, Dept Virol & Immunol, D-37077 Gottingen, Germany

来源：

JOURNAL OF VIROLOGICAL METHODS | 2000年 / 90卷 / 01期

关键词：

polyomavirus; virus-like particle; fluorescence labelling; drug delivery; transporter system; flow cytometry;

D O I：

10.1016/S0166-0934(00)00226-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinantly expressed VP1-virus-like particles (VP1-VLP) of human polyomavirus JC virus (JCV) were described recently as a new DNA transporter system. It was shown that DNA molecules could be packaged into VP1-VLP during a controlled chemical reassociation/dissociation process. In the present study VP1-VLP were studied as carriers for pharmaceutical substances. Propidium iodide (PI) was packaged into VP1-VLP as a reporter molecule. The PI-containing VP1-VLP could be detected directly by flow cytometry. The fluorescence intensity of the VP1-VLP depended strongly on the initial PI concentration. This packaging method is easy to handle and applicable to viruses and VP1-VLP which can be dissociated and reassociated chemically. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：85 / 90

页数：6

共 18 条

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