Expressing genes in different Escherichia coli compartments

被引：76

作者：

Cornelis, P ^{[1
]}

机构：

[1] Free Univ Brussels VIB, Dept Immunol Parasitol & Ultrastruct, Lab Microbial Interact, B-1640 Rhode St Genese, Belgium

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2000年 / 11卷 / 05期

关键词：

D O I：

10.1016/S0958-1669(00)00131-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Production of heterologous proteins or parts thereof in different extra-cytoplasmic compartments (in the periplasm, outer membrane or extracellularly) of Escherichia coli offers multiple applications, for example, in vaccine development, immobilised enzymes and bioremediation. Nowadays, not only surface display of short peptides, but also cell-surface anchoring or secretion of functional proteins is possible. Factors influencing folding, stability and export of extra-cytoplasmic proteins are also better understood.

引用

页码：450 / 454

页数：5

共 51 条

[1]

Åslund F, 1999, J BACTERIOL, V181, P1375

[2] Recombinant protein expression in Escherichia coli [J].

Baneyx, F .

CURRENT OPINION IN BIOTECHNOLOGY, 1999, 10 (05) :411-421

[3] Compatible-solute-supported periplasmic expression of functional recombinant proteins under stress conditions [J].

Barth, S ;

Huhn, M ;

Matthey, B ;

Klimka, A ;

Galinski, EA ;

Engert, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (04) :1572-1579

[4] Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm [J].

Bessette, PH ;

Åslund, F ;

Beckwith, J ;

Georgiou, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (24) :13703-13708

[5] Selection for a periplasmic factor improving phage display and functional periplasmic expression [J].

Bothmann, H ;

Plückthun, A .

NATURE BIOTECHNOLOGY, 1998, 16 (04) :376-380

[6] Expression of foreign antigens on the surface of Escherichia coli by fusion to the outer membrane protein TraT [J].

Chang, HH ;

Sheu, SY ;

Lo, SCJ .

JOURNAL OF BIOMEDICAL SCIENCE, 1999, 6 (01) :64-70

[7] The cystine knot of a squash-type protease inhibitor as a structural scaffold for Escherichia coli cell surface display of conformationally constrained peptides [J].

Christmann, A ;

Walter, K ;

Wentzel, A ;

Krätzner, R ;

Kolmar, H .

PROTEIN ENGINEERING, 1999, 12 (09) :797-806

[8] Development of new cloning vectors for the production of immunogenic outer membrane fusion proteins in Escherichia coli [J].

Cornelis, P ;

Sierra, JC ;

Lim, A ;

Malur, A ;

Tungpradabkul, S ;

Tazka, H ;

Leitao, A ;

Martins, CV ;

diPerna, C ;

Brys, L ;

DeBaetselier, P ;

Hamers, R .

BIO-TECHNOLOGY, 1996, 14 (02) :203-208

[9] A new membrane-bound OprI lipoprotein expression vector High production of heterologous fusion proteins in Gram (-) bacteria and the implications for oral vaccination [J].

Cote-Sierra, J ;

Jongert, E ;

Bredan, A ;

Gautam, DC ;

Parkhouse, M ;

Cornelis, P ;

De Baetselier, P ;

Revets, H .

GENE, 1998, 221 (01) :25-34

[10] Affinity of the periplasmic chaperone Skp of Escherichia coli for phospholipids, lipopolysaccharides and non-native outer membrane proteins -: Role of Skp in the biogenesis of outer membrane protein [J].

de Cock, H ;

Schäfer, U ;

Potgeter, M ;

Demel, R ;

Müller, M ;

Tommassen, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 259 (1-2) :96-103

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