Brain plasticity and cellular mechanisms of epileptogenesis in human and experimental cortical dysplasia

被引:24
作者
Babb, TL
Ying, Z
Mikuni, N
Nishiyama, K
Drazba, J
Bingaman, W
Wyllie, E
Wylie, CJ
Yacubova, K
机构
[1] Cleveland Clin Fdn, Dept Neurosci, Cleveland, OH 44195 USA
[2] Cleveland Clin Fdn, Dept Neurol, Cleveland, OH 44195 USA
[3] Cleveland Clin Fdn, Dept Neurosurg, Cleveland, OH 44195 USA
关键词
central dysplasia; NMDA receptor; gamma radiation; development;
D O I
10.1111/j.1528-1157.2000.tb01561.x
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Purpose: The cellular mechanisms that may contribute to epilepsy in resected human cortical dysplasia (CD) were compared with the in utero radiated rat CD model. In human and rat focal hippocampal epilepsy, postsynaptic N-methyl-D-aspartate receptors are up-regulated and presynaptic axon collaterals hyperinnervate them. We hypothesized that in both human and rat CD: (a) the N-methyl-D-aspartate receptor subunits NR1 and NR2A/B would be increased and coassembled, and (b) aberrant axons would be in regions of CD. Methods: Tests for presynaptic and postsynaptic changes in human and rat CD included the following: (a) cytology, (b) immunocytochemistry, (c) coimmunoprecipitation, (d) double-labeled immunofluorescence, and (e) Timm histochemistry of hippocampal mossy fibers. Within-patient comparisons were made between epileptic tissue, identified by subdural electroencephalographic seizure onsets, and nonepileptic tissue remote from the focus but within the therapeutic resection. Rats were radiated at embryonic day 17, and offspring were studied postnatally. Statistical comparisons were made against normal rats matched for age and tissue processing. Results: In focal CD patients, NR2A/B subunits and their coassemblies with NR1 were increased significantly more than for the remote nonepileptic cortex. Confocal microscopy showed that NR1-NR2A/B colabeled single dysplastic neurons in both human and rat. In CD rats, mossy fibers innervated the anomalously oriented hippocampal neurons. Conclusions: Human epileptic CD exhibits a spectrum of abnormal cell orientations and laminations that must require plastic axodendritic changes during development. These: altered circuits and receptors could account for the seizures and cognitive deficits found in patients with CD. The radiated rat CD model with cortical dyslaminations and NR2A/B subunit increases would allow the development and testing of drugs targeted at only the NR2A/B subunit or at decoupling the NR1-NR2 coassembly, which could provide a specific antiepileptic drug for dysplastic circuits without inducing general depression of all brain neurons.
引用
收藏
页码:S76 / S81
页数:6
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