Discovery of a natural thiamine adenine nucleotide

被引：74

作者：

Bettendorff, Lucien

Wirtzfeld, Barbara

Makarchikov, Alexander F.

Mazzucchelli, Gabriel

Frederich, Michel

Gigliobianco, Tiziana

Gangolf, Marjorie

De Pauw, Edwin

Angenot, Luc

Wins, Pierre

机构：

[1] Univ Liege, Ctr Cellular & Mol Neurobiol, B-4000 Liege, Belgium

[2] Univ Liege, Dept Phys Chem, B-4000 Liege, Belgium

[3] Univ Liege, Drug Res Ctr, Lab Pharmacognosy, B-4000 Liege, Belgium

来源：

NATURE CHEMICAL BIOLOGY | 2007年 / 3卷 / 04期

关键词：

D O I：

10.1038/nchembio867

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several important cofactors are adenine nucleotides with a vitamin as the catalytic moiety. Here, we report the discovery of the first adenine nucleotide containing vitamin B1: adenosine thiamine triphosphate (AThTP, 1), or thiaminylated ATP. We discovered AThTP in Escherichia coli and found that it accumulates specifically in response to carbon starvation, thereby acting as a signal rather than a cofactor. We detected smaller amounts in yeast and in plant and animal tissues.

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页码：211 / 212

页数：2

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共 8 条

[1] Structural basis for transcription regulation by alarmone ppGpp [J].

Artsimovitch, I ;

Patlan, V ;

Sekine, SI ;

Vassylyeva, MN ;

Hosaka, T ;

Ochi, K ;

Yokoyama, S ;

Vassylyev, DG .

CELL, 2004, 117 (03) :299-310

[2] DETERMINATION OF THIAMIN AND ITS PHOSPHATE-ESTERS IN CULTURED NEURONS AND ASTROCYTES USING AN ION-PAIR REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD [J].

BETTENDORFF, L ;

PEETERS, M ;

JOUAN, C ;

WINS, P ;

SCHOFFENIELS, E .

ANALYTICAL BIOCHEMISTRY, 1991, 198 (01) :52-59

[3] A general method for the chemical synthesis of γ-32P-labeled or unlabeled nucleoside 5′-triphosphates and thiamine triphosphate [J].

Bettendorff, L ;

Nghiêm, HO ;

Wins, P ;

Lakaye, B .

ANALYTICAL BIOCHEMISTRY, 2003, 322 (02) :190-197

[4] 2 COMPOUNDS IMPLICATED IN FUNCTION OF RC GENE OF ESCHERICHIA COLI [J].

CHASHEL, M ;

GALLANT, J .

NATURE, 1969, 221 (5183) :838-&

[5] Thiamin biosynthesis in eukaryotes:: Characterization of the enzyme-bound product of thiazole synthase from Saccharomyces cerevisiae and its implications in thiazole biosynthesis [J].

Chatterjee, Abhishek ;

Jurgenson, Christopher T. ;

Schroeder, Frank C. ;

Ealick, Steven E. ;

Begley, Tadhg P. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (22) :7158-7159

[6] Thiamine triphosphate, a new signal required for optimal growth of Escherichia coli during amino acid starvation [J].

Lakaye, B ;

Wirtzfeld, B ;

Wins, P ;

Grisar, T ;

Bettendorff, L .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (17) :17142-17147

[7] Thiamine triphosphate and thiamine triphosphatase activities: from bacteria to mammals [J].

Makarchikov, AF ;

Lakaye, B ;

Gulyai, IE ;

Czerniecki, J ;

Coumans, B ;

Wins, P ;

Grisar, T ;

Bettendorff, L .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2003, 60 (07) :1477-1488

[8] The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography [J].

Wille, Georg ;

Meyer, Danilo ;

Steinmetz, Andrea ;

Hinze, Erik ;

Golbik, Ralph ;

Tittmann, Kai .

NATURE CHEMICAL BIOLOGY, 2006, 2 (06) :324-328