Inhibition of infection caused by severe acute respiratory syndrome-associated coronavirus by equine neutralizing antibody in aged mice

被引：18

作者：

Zhou, Lili

Ni, Bing

Luo, Deyan

Zhao, Guangyu

Jia, Zhengcai

Zhang, Liangyan

Lin, Zhihua

Wang, Li

Zhang, Songle

Xing, Li

Li, Jintao

Liang, Yunfei

Shi, Xinfu

Zhao, TingTing

Zhou, Liyun

Wu, Yuzhang ^{[1
]}

Wang, Xiliang

机构：

[1] Acad Mil Med Sci, State Key Lab Pathogen & Biosecur, Inst Microbiol & Epidemiol, Dept Immunol, Beijing 100071, Peoples R China

[2] Third Mil Med Univ, Inst Immunol, Chongqing 400038, Peoples R China

[3] Chongqing Inst Technol, Coll Bioengn, Chongqing 400050, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2007年 / 7卷 / 03期

基金：

中国国家自然科学基金;

关键词：

SARS-CoV; infection; equine; F(ab')(2); aged; mice;

D O I：

10.1016/j.intimp.2006.10.009

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The high susceptibility of elderly to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) indicates how crucial it is to protect the elderly by various strategies. Aged BALB/c mice displayed a high susceptibility to SARS-CoV and have been a valuable platform for evaluation of strategies against SARS-CoV infection. In this study, we confirmed the validity of this model using various methods, and verified that equine anti-SARS-CoV F(ab')(2) can prevent aged animals from SARS-CoV infection. In a therapeutic setting, treatment with anti-SARS-CoV F(ab')(2) decreased viral load more than several thousand folds in the lungs. Thus, this antibody should be a potential candidate for treatment of elderly patients suffering from SARS. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：392 / 400

页数：9

共 34 条

[1] Severe acute respiratory syndrome (SARS) [J].

G. Bhaskar ;

Rakesh Lodha ;

S. K. Kabra .

The Indian Journal of Pediatrics, 2003, 70 (5) :401-405

[2] Clinical features and short-term outcomes of 144 patients with SARS in the greater Toronto area [J].

Booth, CM ;

Matukas, LM ;

Tomlinson, GA ;

Rachlis, AR ;

Rose, DB ;

Dwosh, HA ;

Walmsley, SL ;

Mazzulli, T ;

Avendano, M ;

Derkach, P ;

Ephtimios, IE ;

Kitai, I ;

Mederski, BD ;

Shadowitz, SB ;

Gold, WL ;

Hawryluck, LA ;

Rea, E ;

Chenkin, JS ;

Cescon, DW ;

Poutanen, SM ;

Detsky, AS .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2003, 289 (21) :2801-2809

[3] Short term outcome and risk factors for adverse clinical outcomes in adults with severe acute respiratory syndrome (SARS) [J].

Chan, JWM ;

Ng, CK ;

Chan, YH ;

Mok, TYW ;

Lee, S ;

Chu, SYY ;

Law, WL ;

Lee, MP ;

Li, PCK .

THORAX, 2003, 58 (08) :686-689

[4] Successful clearance of hepatitis B virus after allogeneic stem cell transplantation: beneficial combination of adoptive immunity transfer and lamivudine [J].

Chiba, T ;

Yokosuka, O ;

Goto, S ;

Fukai, K ;

Imazeki, F ;

Shishido, H ;

Narita, M ;

Saisho, H .

EUROPEAN JOURNAL OF HAEMATOLOGY, 2003, 71 (03) :220-223

[5] Adoptive transfer of immunity: A new strategy to interfere with severe hepatitis virus reinfection after woodchuck liver transplantation [J].

Dahmen, U ;

Dirsch, O ;

Li, J ;

Fiedle, M ;

Lu, M ;

Rispeter, K ;

Picucci, M ;

Broelsch, CE ;

Roggendorf, M .

TRANSPLANTATION, 2004, 77 (07) :965-972

[6] Epidemiological determinants of spread of causal agent of severe acute respiratory syndrome in Hong Kong [J].

Donnelly, CA ;

Ghani, AC ;

Leung, GM ;

Hedley, AJ ;

Fraser, C ;

Riley, S ;

Abu-Raddad, LJ ;

Ho, LM ;

Thach, TQ ;

Chau, P ;

Chan, KP ;

Lam, TH ;

Tse, LY ;

Tsang, T ;

Liu, SH ;

Kong, JHB ;

Lau, EMC ;

Ferguson, NM ;

Anderson, RM .

LANCET, 2003, 361 (9371) :1761-1766

[7] A human SARS-CoV neutralizing antibody against epitope on S2 protein [J].

Duan, JZ ;

Yan, XY ;

Guo, XM ;

Cao, WC ;

Han, W ;

Qi, C ;

Feng, J ;

Yang, DL ;

Gao, GX ;

Jin, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 333 (01) :186-193

[8] Search for SARS origins stalls [J].

Enserink, M ;

Normile, D .

SCIENCE, 2003, 302 (5646) :766-767

[9] An overview of real-time quantitative PCR: Applications to quantify cytokine gene expression [J].

Giulietti, A ;

Overbergh, L ;

Valckx, D ;

Decallonne, B ;

Bouillon, R ;

Mathieu, C .

METHODS, 2001, 25 (04) :386-401

[10] Severe acute respiratory syndrome: Radiographic review of 40 probable cases in Toronto, Canada [J].

Grinblat, L ;

Shulman, H ;

Glickman, A ;

Matukas, L ;

Paul, N .

RADIOLOGY, 2003, 228 (03) :802-809

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