Detection of a specific transposon in Erysiphe necator from grapevines in france

被引：8

作者：

Bouscaut, J. ^{[1
]}

Corio-Costet, M.-F. ^{[1
]}

机构：

[1] INRA, UMR Sante Vegetale, ENITA, ISVV, F-33883 Villenave Dornon, France

来源：

JOURNAL OF PHYTOPATHOLOGY | 2007年 / 155卷 / 06期

关键词：

Erysiphe necator; grape powdery mildew; group A; molecular marker; transposon; Vitis vinifera; UNCINULA-NECATOR; FLAG SHOOT; VINEYARDS; PRIMERS;

D O I：

10.1111/j.1439-0434.2007.01246.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The biotrophic fungus, Erysiphe necator the causal agent of the grape powdery mildew, has two genetic groups A and B in European and Australian vineyards. A strain of group A was used to isolate a DNA sequence that exhibits high sequence homology to RNaseH of a non-LTR (long tandem repeat) retrotransposon of Glomerella cingulata. PCR primers were designed and tested for their specificity to genetic group A of E. necator. This molecular tool is more efficient and sensitive than nested PCR based on polymorphism in the CYP51 and beta-tubulin genes.

引用

页码：381 / 383

页数：3

共 14 条

[1] A single nucleotide polymorphism in the β-tubulin gene distinguishing two genotypes of Erysiphe necator expressing different symptoms on grapevine [J].

Amrani, L. ;

Corio-Costet, M. -F. .

PLANT PATHOLOGY, 2006, 55 (04) :505-512

[2] Genetic similarity of flag shoot and ascospore subpopulations of Erysiphe necator in Italy [J].

Cortesi, P ;

Mazzoleni, A ;

Pizzatti, C ;

Milgroom, MG .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (12) :7788-7791

[3] Transposable elements in filamentous fungi [J].

Daboussi, MJ ;

Capy, P .

ANNUAL REVIEW OF MICROBIOLOGY, 2003, 57 :275-299

[4] A RAPD ASSAY FOR STRAIN TYPING OF THE BIOTROPHIC GRAPE POWDERY MILDEW FUNGUS UNCINULA NECATOR USING DNA EXTRACTED FROM THE MYCELIUM [J].

DELYE, C ;

CORIOCOSTET, MF ;

LAIGRET, F .

EXPERIMENTAL MYCOLOGY, 1995, 19 (03) :234-237

[5]

Délye C, 1999, APPL ENVIRON MICROB, V65, P3950

[6] INITIATION, DEVELOPMENT, DISPERSAL, AND SURVIVAL OF CLEISTOTHECIA OF UNCINULA-NECATOR IN NEW-YORK VINEYARDS [J].

GADOURY, DM ;

PEARSON, RC .

PHYTOPATHOLOGY, 1988, 78 (11) :1413-1421

[7]

Hajjeh H, 2005, J PLANT PATHOL, V87, P71

[8]

He CZ, 1996, MOL GEN GENET, V252, P320

[9] The role of saprotrophy and virulence in the population dynamics of Botrytis cinerea in vineyards [J].

Martinez, F ;

Dubos, B ;

Fermaud, M .

PHYTOPATHOLOGY, 2005, 95 (06) :692-700

[10] THE SIGNIFICANCE OF RESPONSES OF THE GENOME TO CHALLENGE [J].

MCCLINTOCK, B .

SCIENCE, 1984, 226 (4676) :792-801

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