Detection and measurement of a single blood cell surface antigen by thermal lens microscopy

被引：11

作者：

Kimura, H

Nagao, F

Kitamura, A

Sekiguchi, K

Kitamori, T

Sawada, T

机构：

[1] Univ Tokyo, Fac Engn, Dept Appl Chem, Tokyo 1138656, Japan

[2] Juntendo Univ, Sch Med, Dept Forens Med, Bunkyo Ku, Tokyo 113, Japan

[3] Juntendo Univ, Sch Med, Dept Immunol, Bunkyo Ku, Tokyo 113, Japan

来源：

ANALYTICAL BIOCHEMISTRY | 2000年 / 283卷 / 01期

关键词：

thermal lens microscopy; laser spectroscopy; human leukocyte antigen; colloidal gold;

D O I：

10.1006/abio.2000.4614

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive method for detection of antigens on the surface of a single blood cell using thermal lens microscopy is described. Colloidal gold, coated with antibody, was used to stain membrane antigens of leukocytes. Human leukocyte antigens on the lymphocytes and mononuclear leukocytes were observed by new thermal lens microscopy, which involves spectrometry using a laser-induced thermal-lens effect. Antigens of HLA-A, -B, and -C loci on the lymphocytes were identified and quantitated using a single cell. The image of HLA-A, -B, and -C antigen distribution on a mononuclear leukocyte was obtained. Our laser microscope, newly devised for measuring convex surface cells, is a powerful analytical tool for detecting and quantitating localized antigens in a single cell and/or cell-surface-associated molecules. (C) 2000 Academic Press.

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页码：27 / 32

页数：6

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