Amnion-Derived Cellular Cytokine Solution (ACCS) Promotes Migration of Keratinocytes and Fibroblasts

被引：32

作者：

Uberti, M. Georgina ^{[1
]}

Pierpont, Yvonne N. ^{[1
,2
]}

Ko, Francis ^{[1
]}

Wright, Terry E. ^{[1
,2
]}

Smith, Charlotte A. ^{[3
]}

Cruse, C. Wayne ^{[2
]}

Robson, Martin C. ^{[2
]}

Payne, Wyatt G. ^{[1
,2
]}

机构：

[1] Bay Pines VA Healthcare Syst, Inst Tissue Regenerat Repair & Rehabil, Bay Pines, FL USA

[2] Univ S Florida, Dept Surg, Div Plast Surg, Tampa, FL 33620 USA

[3] Stemnion Inc, Pittsburgh, PA USA

来源：

ANNALS OF PLASTIC SURGERY | 2010年 / 64卷 / 05期

关键词：

amnion-derived cellular cytokine solution; wound healing; keratinocyte migration; fibroblast migration; MESENCHYMAL STEM-CELLS; GROWTH-FACTORS; PRESSURE ULCERS; METALLOPROTEINASES; INHIBITORS; MOTILITY; FLUID; PDGF;

D O I：

10.1097/SAP.0b013e3181c39351

中图分类号：

R61 [外科手术学];

学科分类号：

100210 [外科学];

摘要：

Amnion-derived Multipotent Progenitor cells appear to be useful as adjuvants in wound healing. Amnion-derived multipotent progenitor cells secrete a unique combination of cytokines and growth factors, known as amnion-derived cellular cytokine solution (ACCS). In the skin, a cytokine communication network between mesenchymal and epithelial cells tightly controls keratinocyte and fibroblast migration, proliferation and differentiation-key determinants of wound healing. To evaluate the influence of ACCS on the migratory behavior of keratinocytes and fibroblasts, cell migration was assayed quantitatively using a Boyden chamber. Chemotactic migration activity of fibroblasts or keratinocytes through the membrane determined the influence of ACCS. In the presence of ACCS, fibroblasts and keratinocytes demonstrated a statistically significant (P < 0.05) increase in migration when compared with controls. These cell types, critical to normal wound healing, may be influenced to accelerate migration in wounds, thus accelerating wound repair/healing.

引用

页码：632 / 635

页数：4

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