Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction

被引：56

作者：

Huang, FD

Matthies, HJG

Speese, SD

Smith, MA

Broadie, K

机构：

[1] Vanderbilt Univ, Vanderbilt Brain Inst, Vanderbilt Kennedy Ctr, Dept Biol Sci, Nashville, TN 37235 USA

[2] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA

来源：

NATURE NEUROSCIENCE | 2004年 / 7卷 / 10期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nn1313

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.

引用

页码：1070 / 1078

页数：9

共 44 条

[1] InsP(3) receptor is essential for growth and differentiation but not for vision in Drosophila [J].