Immobilization of proteins onto novel, reactive polypyrrole-coated polystyrene latex particles

被引：11

作者：

Bousalem, S

Mangeney, C

Alcote, Y

Chehimi, MA

Basinska, T

Slomkowski, S

机构：

[1] Univ Paris 07, ITODYS, CNRS, UMR 7086, F-75005 Paris, France

[2] Polish Acad Sci, Ctr Mol & Macromol Studies, PL-90363 Lodz, Poland

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2004年 / 249卷 / 1-3期

关键词：

polypyrrole; latex; core/shell particles; reactivity; protein; XPS;

D O I：

10.1016/j.colsurfa.2004.08.056

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Polypyrrole-coated polystyrene latex particles bearing reactive N-ester succinimidyl functional groups (PS-PPyNSE) were prepared by the in situ copolymerization of pyrrole (Py) and the ester-functionalized pyrrole (pyrrole-NSE) in the presence of sterically stabilized polystyrene (PS) latex particles (similar to600 nm). The initial comonomer fractions (in %) were 25/75,40/60,50/50,75/25 and 85/15 for pyrrole-NSE and pyrrole, respectively. The PS-PPyNSE particles were characterized in terms of particle size, surface morphology and surface chemical composition. The reactivity of the particles towards ethylenediamine and human serum albumin (HSA) was examined by FTIR, which unambiguously indicated the formation of interfacial amide groups. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：91 / 94

页数：4

共 7 条

[1]

[Anonymous], HDB CONDUCTING POLYM

[2] Synthesis and characterization of active ester-functionalized polypyrrole-silica nanoparticles: Application to the covalent attachment of proteins [J].