Direct Electrochemistry of Cholesterol Oxidase Immobilized on a Conducting Polymer: Application for a Cholesterol Biosensor

被引：44

作者：

Abdelwahab, Adel A. ^{[1
,2
]}

Won, Mi-Sook ^{[3
]}

Shim, Yoon-Bo ^{[1
,2
]}

机构：

[1] Pusan Natl Univ, Dept Chem, Pusan 609735, South Korea

[2] Pusan Natl Univ, Inst BioPhysio Sensor Technol, Pusan 609735, South Korea

[3] Korea Basic Sci Inst, Pusan 609735, South Korea

来源：

ELECTROANALYSIS | 2010年 / 22卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Direct electrochemistry; Cholesterol oxidase; Microperoxidase; Cholesterol biosensor; Conducting polymer; Biosensors; DIRECT ELECTRON-TRANSFER; CARBON NANOTUBES; GLUCOSE-OXIDASE; MICROPEROXIDASE-11; NANOPARTICLES; HYDRAZINE;

D O I：

10.1002/elan.200900363

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Direct electrochemistry of cholesterol oxidase (ChOx) immobilized on the conductive poly-3',4'-diamine-2,2',5',2 ''-terthiophene (PDATT) was achieved and used to create a cholesterol biosensor. A well-defined redox peak was observed, corresponding to the direct electron transfer of the FAD/FADH(2) of ChOx, and the rate constant (k(s)) was determined to be 0.75 s(-1). Glutathione (GSH) covalently bonded with PDATT was used as a matrix for conjugating AuNPs, ChOx, and MP. simultaneously. MP co-immobilized with ChOx on the AuNPs-GSH/PDATT exhibited an excellent amperometric response to cholesterol. The dynamic range was from 10 to 130 mu M with a detection limit of 0.3 +/- 0.04 mu M.

引用

页码：21 / 25

页数：5

共 27 条

[1] Recent advances in cholesterol biosensor [J].

Arya, Sunil K. ;

Datta, Monika ;

Malhotra, Bansi D. .

BIOSENSORS & BIOELECTRONICS, 2008, 23 (07) :1083-1100

[2] Direct electron transfer of glucose oxidase promoted by carbon nanotubes [J].

Cai, CX ;

Chen, J .

ANALYTICAL BIOCHEMISTRY, 2004, 332 (01) :75-83

[3] Superoxide radical sensing using a cytochrome c3 immobilized conducting polymer electrode [J].

Darain, Farzana ;

Park, Jang-Su ;

Akutsu, Hideo ;

Shim, Yoon-Bo .

BIOSENSORS & BIOELECTRONICS, 2007, 23 (02) :161-167

[4]

Frederickson D.S., 1972, The Metabolic Basis of Inherited Disease, V3rd, P545

[5] Design of an interference-free cholesterol amperometric biosensor based on the electrosynthesis of polymeric films of diaminonaphthalene isomers [J].

García-Ruiz, E ;

Vidal, JC ;

Aramendía, MT ;

Castillo, JR .

ELECTROANALYSIS, 2004, 16 (06) :497-504

[6] Enzyme-catalyzed direct electron transfer: Fundamentals and analytical applications [J].

Ghindilis, AL ;

Atanasov, P ;

Wilkins, E .

ELECTROANALYSIS, 1997, 9 (09) :661-674

[7] Direct electron transfer between heme-containing enzymes and electrodes as basis for third generation biosensors [J].

Gorton, L ;

Lindgren, A ;

Larsson, T ;

Munteanu, FD ;

Ruzgas, T ;

Gazaryan, I .

ANALYTICA CHIMICA ACTA, 1999, 400 :91-108

[8] Fabrication of bienzyme nanobiocomposite electrode using functionalized carbon nanotubes for biosensing applications [J].

Jeykumari, D. R. Shobha ;

Narayanan, S. Sriman .

BIOSENSORS & BIOELECTRONICS, 2008, 23 (11) :1686-1693

[9] Cholesterol biosensor based on electrochemically prepared polyaniline conducting polymer film in presence of a nonionic surfactant [J].

Khan, Raju ;

Solanki, Pratima R. ;

Kaushik, Ajeet ;

Singh, S. P. ;

Ahmad, Sharif ;

Malhotra, B. D. .

JOURNAL OF POLYMER RESEARCH, 2009, 16 (04) :363-373

[10] Design of narrow-bandgap polymers. Syntheses and properties of monomers and polymers containing aromatic-donor and o-quinoid-acceptor units [J].

Kitamura, C ;

Tanaka, S ;

Yamashita, Y .

CHEMISTRY OF MATERIALS, 1996, 8 (02) :570-578

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