Detection of Cryptosporidium oocysts and Giardia cysts in swimming pool filter backwash water concentrates by flocculation and immunomagnetic separation

被引：11

作者：

Greinert, JA

Furtado, DN

Smith, JJ

Barardi, CRM

Simoes, CMO

机构：

[1] Univ Fed Santa Catarina, CCS, CIF, Dept Ciencias Farmaceut, BR-88040900 Florianopolis, SC, Brazil

[2] Univ Fed Santa Catarina, CCB, Dept Microbiol & Parasitol MIP, Lab Virol Aplicada, BR-88040900 Florianopolis, SC, Brazil

[3] Microbiol Serv, Bozeman, MT USA

来源：

INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH | 2004年 / 14卷 / 06期

关键词：

Giardia; Cryptosporidium; swimming pool water; flocculation; immunomagnetic separation;

D O I：

10.1080/09603120400012892

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The purpose of the present study was to evaluate techniques for detection of Giardia cysts and Cryptosporidium oocysts in swimming pool filter backwash water. Calcium carbonate flocculation was used for water samples concentration of 1 l filter backwash water samples. Immunomagnetic separation (IMS) was used for separation of cysts and oocysts from detrital material. Cysts and oocysts were then detected using direct immunofluorescence. ColorSeed C&G(TM) was used as an internal standard. Recoveries were examined at several processing points. Highest recoveries (23% Cryptosporidium , 18% Giardia ) were obtained using lower filter backwash volumes, greater IMS volumes, and addition of Tween20(TM) to backwash samples prior to processing. The combination of CaCO3 flocculation, IMS and direct immunofluorescence was found to be an effective tool for the detection and quantification of Giardia spp. and Cryptosporidium spp. in filter backwash water samples.

引用

页码：395 / 404

页数：10

共 26 条

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