Caenorhabditis elegans SID-2 is required for environmental RNA interference

被引：233

作者：

Winston, William M. ^{[1
]}

Sutherlin, Marie ^{[1
]}

Wright, Amanda J. ^{[1
]}

Feinberg, Evan H. ^{[1
]}

Hunter, Craig P. ^{[1
]}

机构：

[1] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2007年 / 104卷 / 25期

关键词：

dsRNA; transmembrane; intestine lumen;

D O I：

10.1073/pnas.0611282104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

in plants and in the nematode Caenorhabditis elegans, an RNAi signal can trigger gene silencing in cells distant from the site where silencing is initiated. In plants, this signal is known to be a form of dsRNA, and the signal is most likely a form of dsRNA in C elegans as well. Furthermore, in C elegans, dsRNA present in the environment or expressed in ingested bacteria is sufficient to trigger RNAi (environmental RNAi). Ingestion and soaking delivery of dsRNA has also been described for other invertebrates. Here we report the identification and characterization of SID-2, an intestinal luminal transmembrane protein required for environmental RNAi in C elegans. SID-2, when expressed in the environmental RNAi defective species Caenorhabditis briggsae, confers environmental RNAi.

引用

页码：10565 / 10570

页数：6

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