Respiratory syncytial virus decreases the capacity of myeloid dendritic cells to induce interferon-γ in naive T cells

被引:71
作者
Bartz, H [1 ]
Türkel, Ö [1 ]
Hoffjan, S [1 ]
Rothoeft, T [1 ]
Gonschorek, A [1 ]
Schauer, U [1 ]
机构
[1] Ruhr Univ Bochum, St Josef Hosp, Klin Kinder & Jugendmed, D-44791 Bochum, Germany
关键词
D O I
10.1046/j.1365-2567.2003.01629.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis in infants under 6 months of age. Since an RSV infection does not necessarily prevent a reinfection, we asked whether RSV might subvert an effective immune response by interfering with the function of dendritic cells (DCs). Immature DCs cultured from cord blood stem cells and infected with RSV reduced the rate of interferon-gamma (IFN-gamma) production in co-cultured autologous naive T cells stimulated with the superantigen TSST-1. Maturation of DCs in response to poly(IC) but not to CD40 ligand did overcome the inhibitory effect of RSV. Further experiments demonstrated that induction of apoptosis, a selective increase in CD86 expression and lack of release of pro-inflammatory cytokines were associated with inhibition of IFN-gamma generation. In addition, RSV replication seemed to be essential for modulation of IFN-gamma production because a virus preparation inactivated by UV irradiation had no effect. Hence, one reason for multiple reinfections by RSV might be the subversion of antiviral immune responses by interference of RSV with DC function.
引用
收藏
页码:49 / 57
页数:9
相关论文
共 43 条
  • [1] ENHANCED ISOLATION OF RESPIRATORY SYNCYTIAL VIRUS IN CELL-CULTURE
    ARENS, MQ
    SWIERKOSZ, EM
    SCHMIDT, RR
    ARMSTRONG, T
    RIVETNA, KA
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1986, 23 (04) : 800 - 802
  • [2] Respiratory syncytial virus strain A2 is resistant to the antiviral effects of type I interferons and human MxA
    Atreya, PL
    Kulkarni, S
    [J]. VIROLOGY, 1999, 261 (02) : 227 - 241
  • [3] Impaired dendritic cell maturation in patients with chronic, but not resolved, hepatitis C virus infection
    Auffermann-Gratzinger, S
    Keeffe, EB
    Levy, S
    [J]. BLOOD, 2001, 97 (10) : 3171 - 3176
  • [4] Respiratory syncytial virus induces prostaglandin E2, IL-10 and IL-11 generation in antigen presenting cells
    Bartz, H
    Büning-Pfaue, F
    Türkel, Ö
    Schauer, U
    [J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2002, 129 (03) : 438 - 445
  • [5] 2′-5′ oligoadenylate synthetase plays a critical role in interferon-7 inhibition of respiratory syncytial virus infection of human epithelial cells
    Behera, AK
    Kumar, M
    Lockey, RF
    Mohapatra, SS
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (28) : 25601 - 25608
  • [6] VIRUS-INDUCED IMMUNOSUPPRESSION - IMMUNE SYSTEM-MEDIATED DESTRUCTION OF VIRUS-INFECTED DENDRITIC CELLS RESULTS IN GENERALIZED IMMUNE SUPPRESSION
    BORROW, P
    EVANS, CF
    OLDSTONE, MBA
    [J]. JOURNAL OF VIROLOGY, 1995, 69 (02) : 1059 - 1070
  • [7] CD28 function: A balance of costimulatory and regulatory signals
    Bour-Jordan, H
    Bluestone, JA
    [J]. JOURNAL OF CLINICAL IMMUNOLOGY, 2002, 22 (01) : 1 - 7
  • [8] GM-CSF AND TNF-ALPHA COOPERATE IN THE GENERATION OF DENDRITIC LANGERHANS CELLS
    CAUX, C
    DEZUTTERDAMBUYANT, C
    SCHMITT, D
    BANCHEREAU, J
    [J]. NATURE, 1992, 360 (6401) : 258 - 261
  • [9] Maturation, activation, and protection of dendritic cells induced by double-stranded RNA
    Cella, M
    Salio, M
    Sakakibara, Y
    Langen, H
    Julkunen, I
    Lanzavecchia, A
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1999, 189 (05) : 821 - 829
  • [10] INVOLVEMENT OF THE DOUBLE-STRANDED-RNA-DEPENDENT KINASE PKR IN INTERFERON EXPRESSION AND INTERFERON-MEDIATED ANTIVIRAL ACTIVITY
    DER, SD
    LAU, AS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (19) : 8841 - 8845