Column-switching HPLC for the analysis of plasma in PET imaging studies

被引：191

作者：

Hilton, J ^{[1
]}

Yokoi, F ^{[1
]}

Dannals, RF ^{[1
]}

Rauert, HT ^{[1
]}

Szabo, Z ^{[1
]}

Wong, DF ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Radiol, Baltimore, MD 21205 USA

来源：

NUCLEAR MEDICINE AND BIOLOGY | 2000年 / 27卷 / 06期

关键词：

column-switch; HPLC; PET; plasma;

D O I：

10.1016/S0969-8051(00)00125-6

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

A column-switch high performance liquid chromatography method for the analysis of 4 mL, of plasma is described with six examples of chromatography of [C-11] labeled positron emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture column is achieved by disruption of plasma protein binding by 8 M urea. NUCL MED BIOL 27,;6:627-630, 2000. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：627 / 630

页数：4

共 3 条

[1] DISPOSITION OF FORMALDEHYDE AND FORMATE ARISING FROM DRUG N-DEMETHYLATIONS DEPENDENT ON CYTOCHROME-P-450 IN HEPATOCYTES AND IN PERFUSED RAT-LIVER [J].

WAYDHAS, C ;

WEIGL, K ;

SIES, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 89 (01) :143-150

[2] AUTOMATED-DETERMINATION OF DRUGS IN BLOOD-SAMPLES AFTER ENZYMATIC-HYDROLYSIS USING PRECOLUMN SWITCHING AND POST-COLUMN REACTION DETECTION [J].

WERKHOVENGOEWIE, CE ;

DERUITER, C ;

BRINKMAN, UAT ;

FREI, RW ;

DEJONG, GJ ;

LITTLE, CJ ;

STAHEL, O .

JOURNAL OF CHROMATOGRAPHY, 1983, 255 (JAN) :79-90

[3] Evaluation of liquid chromatographic behavior of restricted-access media precolumns in the course of direct injection of large volumes of plasma samples in column-switching systems [J].

Yu, ZX ;

Westerlund, D ;

Boos, KS .

JOURNAL OF CHROMATOGRAPHY B, 1997, 704 (1-2) :53-62

← 1 →