Evidence of immunostimulating lipoprotein existing in the natural lipoteichoic acid fraction

被引:34
作者
Hashimoto, Masahito
Furuyashiki, Maiko
Kaseya, Ryoko
Fukada, Yuka
Akimaru, Mai
Aoyama, Kazue
Okuno, Toshiomi
Tamura, Toshihide
Kirikae, Teruo
Kirikae, Fumiko
Eiraku, Nobutaka
Morioka, Hirofumi
Fujimoto, Yukari
Fukase, Koichi
Takashige, Katsuhiro
Moriya, Yoichiro
Kusumoto, Shoichi
Suda, Yasuo
机构
[1] Kagoshima Univ, Dept Nanostruct & Adv Mat, Grad Sch Sci & Engn, Kagoshima 8900065, Japan
[2] Kagoshima Univ, Hlth Sci Ctr, Kagoshima 8900065, Japan
[3] Int Med Ctr Japan, Dept Infect Dis, Tokyo 1628655, Japan
[4] Osaka Univ, Grad Sch Sci, Dept Chem, Osaka 5600043, Japan
[5] Chugai Pharmaceut Co Ltd, Kanagawa 2478530, Japan
关键词
D O I
10.1128/IAI.02083-05
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Lipoteichoic acid (LTA) is a cell surface glycoconjugate of gram-positive bacteria and is reported to activate the innate immune system. We previously reported that purified LTA obtained from Enterococcus hirae has no immunostimulating activity, but a subfraction (Eh-AF) in an LTA fraction possesses activity. In this study, we established a mouse monoclonal antibody neutralizing the activity of Eh-AF and investigated its inhibitory effects. Monoclonal antibody (MAbEh1) was established by the immunization of BALB/c mice with Eh-AF, followed by hybridoma screening based on its inhibitory effect for the production of interleukin-6 (IL-6) induced by Eh-AF. M.AbEh1 neutralized the production of IL-6 by LTA fraction from not only E. hirae but also Staphylococcus aureus, while it failed to block that of lipopolysaccharide, suggesting that the antibody recognized a common active structure(s) in LTA fractions. Synthetic glycolipids in these LTAs did not induce cytokine production, at least in our system. Interestingly, the antibody was found to inhibit the activity of immunostimulating synthetic lipopeptides, Pam(3)CSK(4) and FSL-1. These results suggest that MAbEh1 neutralizes the activity of lipoprotein-like compounds which is responsible for the activity of the LTA fraction of E. hirae and S. aureus.
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页码:1926 / 1932
页数:7
相关论文
共 36 条
[1]   Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3 [J].
Alexopoulou, L ;
Holt, AC ;
Medzhitov, R ;
Flavell, RA .
NATURE, 2001, 413 (6857) :732-738
[2]   THE STRUCTURE OF PNEUMOCOCCAL LIPOTEICHOIC ACID - IMPROVED PREPARATION, CHEMICAL AND MASS-SPECTROMETRIC STUDIES [J].
BEHR, T ;
FISCHER, W ;
PETERKATALINIC, J ;
EGGE, H .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 207 (03) :1063-1075
[3]   STIMULATION OF MONOKINE PRODUCTION BY LIPOTEICHOIC ACIDS [J].
BHAKDI, S ;
KLONISCH, T ;
NUBER, P ;
FISCHER, W .
INFECTION AND IMMUNITY, 1991, 59 (12) :4614-4620
[4]  
Fischer W., 1990, GLYCOLIPIDS PHOSPHOG, P123
[5]   SYNTHETIC STUDY OF LIPOTEICHOIC ACID OF GRAM-POSITIVE BACTERIA .2. SYNTHESIS OF THE PROPOSED FUNDAMENTAL STRUCTURE OF ENTEROCOCCUS-HIRAE LIPOTEICHOIC ACID [J].
FUKASE, K ;
YOSHIMURA, T ;
KOTANI, S ;
KUSUMOTO, S .
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1994, 67 (02) :473-482
[6]   SYNTHETIC STUDY ON LIPOTEICHOIC ACID OF GRAM-POSITIVE BACTERIA .1. SYNTHESIS OF PROPOSED FUNDAMENTAL STRUCTURE OF STREPTOCOCCUS-PYOGENES LIPOTEICHOIC ACID [J].
FUKASE, K ;
MATSUMOTO, T ;
ITO, N ;
YOSHIMURA, T ;
KOTANI, S ;
KUSUMOTO, S .
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1992, 65 (10) :2643-2654
[7]   Pneumococcal lipoteichoic acid (LTA) is not as potent as staphylococcal LTA in stimulating toll-like receptor 2 [J].
Han, SH ;
Kim, JH ;
Martin, M ;
Michalek, SM ;
Nahm, MH .
INFECTION AND IMMUNITY, 2003, 71 (10) :5541-5548
[8]  
Hashimoto K, 2006, J HIGH ENERGY PHYS
[9]   Separation and structural analysis of lipoprotein in a lipopolysaccharide preparation from Porphyromonas gingivalis [J].
Hashimoto, M ;
Asai, Y ;
Ogawa, T .
INTERNATIONAL IMMUNOLOGY, 2004, 16 (10) :1431-1437
[10]  
Hashimoto M, 1997, J BIOCHEM-TOKYO, V121, P779