Urinary excretion of purine derivatives as an index of microbial-nitrogen intake in growing rabbits

被引:14
作者
Balcells, J [1 ]
Ganuza, JM [1 ]
Pérez, JF [1 ]
Martín-Orúe, SM [1 ]
Ronquillo, MG [1 ]
机构
[1] Fac Vet, Dept Prod Anim & Ciencia Alimentos, Zaragoza 50013, Spain
关键词
purines; xanthine oxidase; rabbit;
D O I
10.1079/BJN19980062
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Three experiments were carried out to establish a response model between intake and urinary excretion of purine compounds. In Expt 1 the relationship between the intake of purine bases (PB) and the excretion of total purine derivatives (PD) was determined in seven growing rabbits with a mean initial live weight (LW) of 2.03 (SE 0.185) kg, aged 70 d, each fitted with a wooden neck collar to prevent caecotrophagy. They were fed on five experimental diets formulated with different levels of nucleic acids (0.00, 3.75, 7.50, 11.25, 15.00 g yeast-RNA/kg diet). The relationship between intake of purine (x, mu mol/kg W-0.75) and total urinary PD excretion (y, mu mol/kg W-0.75), y = 0.56 + 0.67x (r(2) 0.86; RSD 0.338), indicated that about 70 % of duodenal PB were recovered as urinary PD and that the endogenous contribution was constant and independent of dietary PB supply. Endogenous excretion of PD (allantoin and uric acid) was measured in a second experiment using six rabbits fed on a purine-free diet and fitted with neck collars to avoid caecotrophagy. Basal daily urinary excretion Values for allantoin and uric acid were 532 (SE 33.9) and 55 (SE 7.3) mu mol/kg W-0.75 respectively; xanthine and hypoxanthine were not found in urine samples and therefore the sum of allantoin and uric acid should comprise the total excretion of PD (588 (SE 7.3) mu mol/kg W-0.75). The xanthine oxidase (EC 1.2.3.2) activity in plasma, liver, duodenum, jejunum and kidney was measured in a third experiment. The activities of xanthine oxidase in duodenal and jejunal mucosa, liver and kidney were: 0.61 (SE 0.095), 0.37 (SE 0.045), 0.035 (SE 0.001) and 0 units/g fresh tissue respectively and in plasma 2.96 (SE 0.094) units/l. The results show that urinary excretion of PD may be a useful tool to estimate duodenal PB input and microbial protein intake once the relationship between PB and N has been established in caecal micro-organisms.
引用
收藏
页码:373 / 380
页数:8
相关论文
共 37 条
  • [1] SPECIES DISTRIBUTION OF XANTHINE OXIDASE
    ALKHALIDI, UA
    CHAGLASSIAN, TH
    [J]. BIOCHEMICAL JOURNAL, 1965, 97 (01) : 318 - +
  • [2] URINARY EXCRETIONS OF PURINE DERIVATIVES AND NITROGEN IN SHEEP GIVEN STRAW SUPPLEMENTED WITH DIFFERENT SOURCES OF CARBOHYDRATES
    BALCELLS, J
    FONDEVILA, M
    GUADA, JA
    CASTRILLO, C
    SURRA, JCE
    [J]. ANIMAL PRODUCTION, 1993, 57 : 287 - 292
  • [3] URINARY-EXCRETION OF ALLANTOIN AND ALLANTOIN PRECURSORS BY SHEEP AFTER DIFFERENT RATES OF PURINE INFUSION INTO THE DUODENUM
    BALCELLS, J
    GUADA, JA
    CASTRILLO, C
    GASA, J
    [J]. JOURNAL OF AGRICULTURAL SCIENCE, 1991, 116 : 309 - 317
  • [4] SIMULTANEOUS DETERMINATION OF ALLANTOIN AND OXYPURINES IN BIOLOGICAL-FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    BALCELLS, J
    GUADA, JA
    PEIRO, JM
    PARKER, DS
    [J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 575 (01): : 153 - 157
  • [5] Consequences of reduced fibre intake on digestion, rate of passage and caecal microbial activity in the young rabbit
    Bellier, R
    Gidenne, T
    [J]. BRITISH JOURNAL OF NUTRITION, 1996, 75 (03) : 353 - 363
  • [6] SECRETION OF PURINES BY SMALL INTESTINE - GENERAL CHARACTERISTICS
    BERLIN, RD
    HAWKINS, RA
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1968, 215 (04): : 932 - &
  • [7] Box G.E., 1978, STAT EXPT
  • [8] CULTURAL METHODS AND SOME CHARACTERISTICS OF SOME OF THE MORE NUMEROUS GROUPS OF BACTERIA IN THE BOVINE RUMEN
    BRYANT, MP
    BURKEY, LA
    [J]. JOURNAL OF DAIRY SCIENCE, 1953, 36 (03) : 205 - 217
  • [9] CARABANO R, 1988, J ANIM SCI, V66, P901
  • [10] CHANEY AL, 1962, CLIN CHEM, V8, P130