Evaluation of dried whole blood spots obtained by heel or finger stick as an alternative to venous blood for diagnosis of human immunodeficiency virus type 1 infection in vertically exposed infants in the routine diagnostic laboratory

被引：46

作者：

Patton, Janet C.

Akkers, Eveline

Coovadia, Ashraf H.

Meyers, Tammy M.

Stevens, Wendy S.

Sherman, Gayle G.

机构：

[1] Univ Witwatersrand, Wits Pediat HIV Clin, Wits Hlth Consortium, Johannesburg, Gauteng, South Africa

[2] Univ Witwatersrand, Dept Mol Med & Hematol, Johannesburg, Gauteng, South Africa

[3] Univ Witwatersrand, Dept Pediat, Johannesburg, Gauteng, South Africa

[4] Natl Hlth Lab Serv, Johannesburg, Gauteng, South Africa

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2007年 / 14卷 / 02期

关键词：

D O I：

10.1128/CVI.00223-06

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The diagnostic accuracy of the Roche Amplicor human immunodeficiency virus type 1 DNA PCR assay (version 1.5) on DNA extracted from pediatric heel prick dried blood spots using Roche MagNA Pure nucleic acid purification technology was evaluated. The methodologies transfer successfully from the labor-intensive research laboratory to the high-throughput automated routine laboratory.

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页码：201 / 203

页数：3

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