In vivo incorporation of an alkyne into proteins in Escherichia coli

被引：141

作者：

Deiters, A

Schultz, PG

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2005年 / 15卷 / 05期

关键词：

D O I：

10.1016/j.bmcl.2004.12.065

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Using a genetic selection we identified mutants of the M. janaschii tyrosyl-tRNA synthetase that selectively charge an amber suppressor tRNA with para-propargyloxyphenylalanine in Escherichia coli. These evolved tRNA-synthetase pairs were used to site-specifically incorporate an alkynyl group into a protein, which was subsequently conjugated with fluorescent dyes by a [3+2]-cycloaddition reaction under mild reaction conditions. (c) 2004 Elsevier Ltd. All rights reserved.

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页码：1521 / 1524

页数：4

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