HPLC-based activity profiling of Salvia miltiorrhiza for MAO A and iNOS inhibitory activities

被引：37

作者：

Dittmann, K

Gerhäuser, C

Klimo, K

Hamburger, M

机构：

[1] Univ Basel, Inst Pharmaceut Biol, Dept Pharmaceut Sci, CH-4056 Basel, Switzerland

[2] Univ Jena, Inst Pharm, D-6900 Jena, Germany

[3] German Canc Res Ctr, DKFZ, D-6900 Heidelberg, Germany

来源：

PLANTA MEDICA | 2004年 / 70卷 / 10期

关键词：

Salvia miltiorrhiza; Lamiaceae; diterpenoids; tanshinones; HPLC-DAD-MS; MAO A inhibition; inhibition of iNOS induction; RAW; 267.4; cells;

D O I：

10.1055/s-2004-832615

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In the course of a screening of plant extracts for potential CNS and anti-inflammatory activities, a dichloromethane extract of Salvia miltiorrhiza showed a pronounced inhibitory effect on recombinant monoamine oxidase A (MAO A) and on inducible NO synthase (NOS) induction in Raw 267.4 cells. With the aid of HPLC-based profiling techniques, activities could be linked, to a significant extent, to peaks of tanshinone-type diterpenoids, four of which were subsequently isolated. The IC50 of the most active compound, 15,16-dihydrotanshinone 1, on human recombinant MAO A was at 23 muM, and 2.4 muM on lipopolysaccharide-mediated iNOS induction in Raw 267.4 cells.

引用

页码：909 / 913

页数：5

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