Use of species-directed 16S rRNA gene PCR primers for detection of Atopobium vaginae in patients with bacterial vaginosis

被引：20

作者：

Ferris, MJ

Masztal, A

Martin, DH

机构：

[1] Louisiana State Univ, Ctr Hlth Sci, Dept Pediat, New Orleans, LA 70112 USA

[2] Louisiana State Univ, Ctr Hlth Sci, Dept Internal Med, New Orleans, LA 70112 USA

[3] Res Inst Children, New Orleans, LA USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 12期

关键词：

D O I：

10.1128/jcm.42.12.5892-5894.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recent studies suggest that the association between a metrouidazole-resistant anaerobe, Atopobium vaginae, and bacterial vaginosis (BV) warrants further investigation. In the present study, specific primers enhanced detection of A. vaginae and provided additional evidence that this bacterium is prevalent among patients with BV but absent among patients with normal vaginal flora.

引用

收藏

页码：5892 / 5894

页数：3

相关论文

共 27 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database [J].

Ashelford, KE ;

Weightman, AJ ;

Fry, JC .

NUCLEIC ACIDS RESEARCH, 2002, 30 (15) :3481-3489

[3] Detection of Atopobium vaginae in postmenopausal worrien by cultivation-independent methods warrants further investigation [J].

Burton, JP ;

Devillard, E ;

Cadieux, PA ;

Hammond, JA ;

Reid, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (04) :1829-1831

[4] Association between bacterial vaginosis and expression of human immunodeficiency virus type 1 RNA in the female genital tract [J].

Cu-Uvin, S ;

Hogan, JW ;

Caliendo, AM ;

Harwell, J ;

Mayer, KH ;

Carpenter, CCJ .

CLINICAL INFECTIOUS DISEASES, 2001, 33 (06) :894-896

[5] Association of Atopobium vaginae, a recently described metronidazole resistant anaerobe, with bacterial vaginosis -: art. no. 5 [J].

Ferris, MJ ;

Masztal, A ;

Aldridge, KE ;

Fortenberry, D ;

Fidel, PL ;

Martin, DH .

BMC INFECTIOUS DISEASES, 2004, 4 (1)

[6] Cyanobacterial ecotypes in different optical microenvironments of a 68°C hot sring mat community revealed by 16S-23S rRNA internal transcribed spacer region variation [J].

Ferris, MJ ;

Kühl, M ;

Wieland, A ;

Ward, DM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (05) :2893-2898

[7]

Hillier S, 1999, Sexually transmitted diseases, P563

[8] Association between bacterial vaginosis and preterm delivery of a low-birth-weight infant [J].

Hillier, SL ;

Nugent, RP ;

Eschenbach, DA ;

Krohn, MA ;

Gibbs, RS ;

Martin, DH ;

Cotch, MF ;

Edelman, R ;

Pastorek, JG ;

Rao, AV ;

McNellis, D ;

Regan, JA ;

Carey, JC ;

Klebanoff, MA .

NEW ENGLAND JOURNAL OF MEDICINE, 1995, 333 (26) :1737-1742

[9] Longitudinal analysis of bacterial vaginosis: Findings from the HIV epidemiology research study [J].

Jamieson, DJ ;

Duerr, A ;

Klein, RS ;

Paramsothy, P ;

Brown, W ;

Cu-Uvin, S ;

Rompalo, A ;

Sobel, J .

OBSTETRICS AND GYNECOLOGY, 2001, 98 (04) :656-663

[10] High rate of bacterial vaginosis among women with intrauterine devices in Manado, Indonesia [J].

Joesoef, MR ;

Karundeng, A ;

Runtupalit, C ;

Moran, JS ;

Lewis, JS ;

Ryan, CA .

CONTRACEPTION, 2001, 64 (03) :169-172